精子DNA损伤与试管婴幼儿结局的相关性分析
|
摘要
目的探讨精子DNA损伤对试管婴幼儿结局的影响。方法回顾分析2015年3月至2017年12月我生殖专科的62例进行试管婴儿辅助生殖者,按DNA损伤情况分为损伤组与正常组,每组各31例,对比两组精子各项指标情况与试管婴幼儿结局。结果①损伤组精子浓度、精子活力、精子正常形态、顶体酶、IVF受精率、受精率、优质胚胎率均明显高于损伤组(P<0.05),正常组ICSI受精率明显低于损伤组(P<0.05);②正常组活产率为(61.29%)明显高于对照组(32.25%)(P<0.05),两组流产率无明显差异(P>0.05)。结论精子DNA损伤监测可作为评估实施试管婴儿前重要的一项指标,采取适当的方式减少精子DNA的损伤,可提高试管婴儿的成功率。
Objective To investigate the effect of sperm DNA damage on the outcome of infants in vitro. Methods A retrospective analysis of 62 cases of IVF assisted reproductive workers from March 2015 to December 2017 was conducted. The patients were divided into injury group and normal group according to the DNA damage. Each group had 31 cases, and the sperm indexes of the two groups were compared. The situation is the end of the test tube infants. Results Firstly, the sperm concentration, sperm motility, normal sperm morphology, acrosome enzyme, IVF fertilization rate, fertilization rate and high quality embryo rate were significantly higher in the injured group than in the injury group(P<0.05). The ICSI fertilization rate in the normal group was significantly lower than that in the injury group,(P<0.05). Secondly, the live birth rate of the normal group was(61.29%) significantly higher than that of the control group(32.25%)(P<0.05). There was no significant difference in the abortion rate between the two groups(P>0.05). Conclusion Sperm DNA damage monitoring can be used as an important indicator to evaluate the implementation of IVF. Appropriate ways to reduce sperm DNA damage can improve the success rate of test tube baby.
Objective To investigate the effect of sperm DNA damage on the outcome of infants in vitro. Methods A retrospective analysis of 62 cases of IVF assisted reproductive workers from March 2015 to December 2017 was conducted. The patients were divided into injury group and normal group according to the DNA damage. Each group had 31 cases, and the sperm indexes of the two groups were compared. The situation is the end of the test tube infants. Results Firstly, the sperm concentration, sperm motility, normal sperm morphology, acrosome enzyme, IVF fertilization rate, fertilization rate and high quality embryo rate were significantly higher in the injured group than in the injury group(P<0.05). The ICSI fertilization rate in the normal group was significantly lower than that in the injury group,(P<0.05). Secondly, the live birth rate of the normal group was(61.29%) significantly higher than that of the control group(32.25%)(P<0.05). There was no significant difference in the abortion rate between the two groups(P>0.05). Conclusion Sperm DNA damage monitoring can be used as an important indicator to evaluate the implementation of IVF. Appropriate ways to reduce sperm DNA damage can improve the success rate of test tube baby.
引文
[1]姚佳欣,陈诗言,刘青松,等.精子细胞XRCC1基因rs25487位点多态性与少弱精患者的相关性研究[J].西部医学,2018,29(1):156-157.
[2]邵淑敏,李姣,邹敏,等.精子DNA损伤对IVF/ICSI-ET结局影响的回顾性资料分析[J].中国计划生育学杂志,2016,24(3):179-182.
[3]宋焱鑫,郭燕京,董波,等.精子DNA碎片指数阈值与体外受精-胚胎移植结局的相关性研究[J].生殖医学杂志,2017,26(6):546-551.
[4]龙晓宇,乔杰.精准医疗在生殖医学临床中的应用[J].实用妇产科杂志,2017,33(6):407-408.
[5]李焕,赵军,邓浩,等.腹膜后显微镜精索静脉结扎术后精子DNA碎片率变化[J].医学分子生物学杂志,2017,14(6):347-351.
[6]刘太秀,崔志鸿,曹佳.重庆市大学生精液常规参数与精子DNA损伤的关联性分析[J].第三军医大学学报,2016,38(11):1325-1330.
[7]杨纯,耿育红,高颖.赠卵试管婴儿临床结局及其影响因素的研究[J].生殖医学杂志,2016,25(3):209-213.
[8]麦选诚,董云华,陈斌,等.不育患者精子DNA损伤和精液常规参数关系分析[J].中国男科学杂志,2016,30(4):19-22.
[9]叶莹心,曲陆荣,于阳,等.赠卵试管婴儿1例分娩报告[J].中国实用妇科与产科杂志,2000(10):58.
[10]罗桂英,王蒙蒙,王春艳,等.179例试管婴儿孕妇发生先兆流产的病例对照研究[J].安徽医科大学学报,2015,50(11):1634-1638.
[11]邱雪芳,陈胜莲,陈汝仙,等.试管婴儿技术中患者心理情况及护理效果评价[J].实用预防医学,2013,20(06):728-730.
[2]邵淑敏,李姣,邹敏,等.精子DNA损伤对IVF/ICSI-ET结局影响的回顾性资料分析[J].中国计划生育学杂志,2016,24(3):179-182.
[3]宋焱鑫,郭燕京,董波,等.精子DNA碎片指数阈值与体外受精-胚胎移植结局的相关性研究[J].生殖医学杂志,2017,26(6):546-551.
[4]龙晓宇,乔杰.精准医疗在生殖医学临床中的应用[J].实用妇产科杂志,2017,33(6):407-408.
[5]李焕,赵军,邓浩,等.腹膜后显微镜精索静脉结扎术后精子DNA碎片率变化[J].医学分子生物学杂志,2017,14(6):347-351.
[6]刘太秀,崔志鸿,曹佳.重庆市大学生精液常规参数与精子DNA损伤的关联性分析[J].第三军医大学学报,2016,38(11):1325-1330.
[7]杨纯,耿育红,高颖.赠卵试管婴儿临床结局及其影响因素的研究[J].生殖医学杂志,2016,25(3):209-213.
[8]麦选诚,董云华,陈斌,等.不育患者精子DNA损伤和精液常规参数关系分析[J].中国男科学杂志,2016,30(4):19-22.
[9]叶莹心,曲陆荣,于阳,等.赠卵试管婴儿1例分娩报告[J].中国实用妇科与产科杂志,2000(10):58.
[10]罗桂英,王蒙蒙,王春艳,等.179例试管婴儿孕妇发生先兆流产的病例对照研究[J].安徽医科大学学报,2015,50(11):1634-1638.
[11]邱雪芳,陈胜莲,陈汝仙,等.试管婴儿技术中患者心理情况及护理效果评价[J].实用预防医学,2013,20(06):728-730.