慢性HIV感染者外周血T细胞CD28、CD160表达水平的研究
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摘要
目的探讨CD28、CD160在慢性人类免疫缺陷病毒(HIV)感染者体内的表达及其临床意义。方法选取2016年1月至2017年1月收治的50例HIV感染患者,作为观察组,并征集50例健康志愿者,作为对照组。观察并记录所有研究参与者一般资料、CD4+、CD8+T细胞上CD160及CD28的表达及初始T细胞(TN)、中央记忆T细胞(TCM)、效应记忆T细胞(TEM)、终末效应记忆T细胞(TEMRA)亚群上CD160表达的百分比及平均荧光强度(MFI)、两种细胞上病毒载量,并分析CD28及CD160的表达水平与患者CD4+T细胞计数和病毒载量的相关性。结果随着CD4+CD160的表达增加,CD4+T细胞呈下降趋势,两者呈负相关(r=-0.561,P<0.05),CD8+T细胞数量呈上升趋势,两者呈正相关(r=0.619,P<0.05),而HIV-RNA拷贝数量随着CD4+T细胞上、CD8+T细胞上CD160表达的增加而增加,两者呈正相关(r=0.684,P<0.05、r=0.459,P<0.05);随着CD4+T细胞上CD28的表达增加,CD4+、CD8+T细胞计数呈上升趋势,两者呈正相关(r=0.621,P<0.05、r=0.527,P<0.05),而HIV-RNA拷贝数量随着CD4+、CD8+T细胞上CD28表达的增加而降低,两者呈负相关(r=-0.634,P<0.05、r=-0.582,P<0.05);两组患者CD8+T细胞CD160在TEMRA亚群表达阳性率及MFI差异无统计学意义(P>0.05),观察组患者CD8+T细胞CD160在TN、TCM、TEM亚群表达阳性率及MFI均高于对照组,差异有统计学意义(P<0.05)。结论慢性HIV感染患者中CD28表达有所减少,而CD160表达有所增加,可能与HIV患者CD4+T、CD8+T细胞减少有关,其中CD160主要影响记忆性CD8+T细胞。
Objective To investigate the expression and clinical significance of CD28 and CD160 in patients with chronic HIV infection.Methods 50 patients with HIV from January 2016 to January 2017 were selected as the observation group,and 50 healthy volunteers were recruited as control group.Observe and record general information of all participants,the expression of CD28,CD160 in CD4+and CD8+T cells,initial T cells(TN),the expression of CD160 in central memory T cells(TCM),effector memory T cells(TEM),end effector memory T cells(TEMRA),mean fluorescence intensity(MFI),viral load of two kinds of the cells,analyze the correlation between the expression level of CD28 and CD160 and CD4+T cell count and viral load.Results With the increase of CD160 expression of CD4+T cells,CD4+T cells showed a downward trend,there is a negative correlation between them(r=-0.561,P<0.05),CD8+T cell number is on the rise,there is a positive correlation between them(r=0.619,P<0.05),and HIV-RNA copy number increased with the increase of CD160 expression on CD4+T cells and CD8+T cells,both positive(r=0.684,P<0.05,r=0.459,P<0.05);with the increase of CD28 cells on the expression of CD4+T,CD4+,CD8+T cells showed a rising trend,there is a positive correlation between them(r=0.621,P<0.05,r=0.527,P<0.05,HIV-RNA)and the copy number decreased with the increase of the expression of CD28 and CD4+T on CD8+T cells,there is a negative correlation between them(r=-0.634,P<0.05,r=-0.582,P<0.05).There was no significant difference in the positive rate of expression in TEMRA subgroup and MFI of CD160 in CD8+T cell in two groups(P>0.05).The positive rate and MFI of CD8+T cell CD160 in TN,TCM and TEM subgroups in observation group were significantly higher than those in control group(Tcm),with statistical significance.Conclusion The expression of CD28 in patients with chronic HIV infection is decreased,and the expression of CD160 is increased,which may be related to the decrease of HIV CD4+T and CD8+T cells,in which CD160 mainly affects the memory CD8+T.
Objective To investigate the expression and clinical significance of CD28 and CD160 in patients with chronic HIV infection.Methods 50 patients with HIV from January 2016 to January 2017 were selected as the observation group,and 50 healthy volunteers were recruited as control group.Observe and record general information of all participants,the expression of CD28,CD160 in CD4+and CD8+T cells,initial T cells(TN),the expression of CD160 in central memory T cells(TCM),effector memory T cells(TEM),end effector memory T cells(TEMRA),mean fluorescence intensity(MFI),viral load of two kinds of the cells,analyze the correlation between the expression level of CD28 and CD160 and CD4+T cell count and viral load.Results With the increase of CD160 expression of CD4+T cells,CD4+T cells showed a downward trend,there is a negative correlation between them(r=-0.561,P<0.05),CD8+T cell number is on the rise,there is a positive correlation between them(r=0.619,P<0.05),and HIV-RNA copy number increased with the increase of CD160 expression on CD4+T cells and CD8+T cells,both positive(r=0.684,P<0.05,r=0.459,P<0.05);with the increase of CD28 cells on the expression of CD4+T,CD4+,CD8+T cells showed a rising trend,there is a positive correlation between them(r=0.621,P<0.05,r=0.527,P<0.05,HIV-RNA)and the copy number decreased with the increase of the expression of CD28 and CD4+T on CD8+T cells,there is a negative correlation between them(r=-0.634,P<0.05,r=-0.582,P<0.05).There was no significant difference in the positive rate of expression in TEMRA subgroup and MFI of CD160 in CD8+T cell in two groups(P>0.05).The positive rate and MFI of CD8+T cell CD160 in TN,TCM and TEM subgroups in observation group were significantly higher than those in control group(Tcm),with statistical significance.Conclusion The expression of CD28 in patients with chronic HIV infection is decreased,and the expression of CD160 is increased,which may be related to the decrease of HIV CD4+T and CD8+T cells,in which CD160 mainly affects the memory CD8+T.
引文
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[11]郑海清,陈壮桂,刘秋莉,等.CD8+CD28-T淋巴细胞在骨髓间充质干细胞治疗实验性自身免疫性脑脊髓炎小鼠中的免疫调节作用[J].中华物理医学与康复杂志,2016,38(9):641-646.
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[13]肖晗,孙红,刘秀珍,等.CD160的表达与荷瘤小鼠CD8+T细胞免疫活性的关系[J].中华实验外科杂志,2015,32(7):1653-1656.
[14]王利伟,任伟宏.HIV/AIDS患者CD8+T淋巴细胞CD28、CD38分子表达及其与疾病的相关性研究[J].检验医学与临床,2016(3):404-406.
[15]王陆军,徐向升,冯国华,等.共抑制分子CD160在慢性HIV-1感染患者中的表达及临床意义[J].中华医学杂志,2014,94(20):1559-1562.
[2]杨国强.HIV/HCV共感染者T淋巴细胞亚群CD28,CD38分子表达特征的分析[J].中国医药指南,2016,14(7):5-6.
[3]谢静,邱志峰,韩扬,等.未经抗病毒治疗的HIV/AIDS患者CD8+T细胞HLA-DR,CD38表达水平与CD4+T细胞计数的相关性研究[J].中华内科杂志,2016,55(1):36-39.
[4] LI N,JI P,SONG L G,et al.The expression of molecule CD28and CD38on CD4+/CD8+T lymphocytes in thymus and spleen elicited by Schistosoma japonicum infection in mice model[J].Parasitol Res,2015,114(8):3047-3058.
[5]吴敏娟.CD4+CD25+Treg和CD4+PD-1+T细胞亚群在HIV感染者/艾滋病人外周血的分布及其临床意义[D].苏州:苏州大学,2015.
[6]崔小健.HIV感染者NK细胞介导的ADCC功能变化研究[D].沈阳:中国医科大学,2013.
[7]葛均波,徐永健.内科学[M].8版.北京:人民卫生出版社,2014:212-214.
[8] FIGUEIREDO S,CHARMETEAU B,SURENAUD M,et al.Memory CD8(+)T cells elicited by HIV-1lipopeptide vaccines display similar phenotypic profiles but differences in term of magnitude and multifunctionality compared with FLU-or EBV-specific memory T cells in humans[J].Vaccine,2014,32(4):492-501.
[9] ECHEVERRA A,MORO-GARCA M A,ASENSI V,et al.CD4+CD28null T lymphocytes resemble CD8+CD28null T lymphocytes in their responses to IL-15and IL-21in HIV-infected patients[J].J Leukoc Biol,2015,98(3):373-384.
[10]CILLO A R,SOBOLEWSKI M D,BOSCH R J,et al.Quantification of HIV-1latency reversal in resting CD4+Tcellsfrompatientsonsuppressive antiretroviral therapy[J].Proc Natl Acad Sci U S A,2014,111(19):7078-7083.
[11]郑海清,陈壮桂,刘秋莉,等.CD8+CD28-T淋巴细胞在骨髓间充质干细胞治疗实验性自身免疫性脑脊髓炎小鼠中的免疫调节作用[J].中华物理医学与康复杂志,2016,38(9):641-646.
[12]DUVERGER A,WOLSCHENDORF F,ANDERSON J C,et al.Kinase control of latent HIV-1infection:PIM-1kinase as a major contributor to HIV-1reactivation[J].J Virol,2014,88(1):364-376.
[13]肖晗,孙红,刘秀珍,等.CD160的表达与荷瘤小鼠CD8+T细胞免疫活性的关系[J].中华实验外科杂志,2015,32(7):1653-1656.
[14]王利伟,任伟宏.HIV/AIDS患者CD8+T淋巴细胞CD28、CD38分子表达及其与疾病的相关性研究[J].检验医学与临床,2016(3):404-406.
[15]王陆军,徐向升,冯国华,等.共抑制分子CD160在慢性HIV-1感染患者中的表达及临床意义[J].中华医学杂志,2014,94(20):1559-1562.