牛磺酸对MDCC-MSB1细胞端粒酶表达水平及其活性的影响
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摘要
为探讨牛磺酸对鸡MDCC-MSB1肿瘤细胞系增殖的影响及其可能的作用机理,研究在牛磺酸应用适宜浓度范围内,使用添加0%、0.5%、1.0%、2.0%牛磺酸的RPMI1640培养基,培养MDCCMSB1细胞系,探讨牛磺酸对鸡MD肿瘤细胞chTERT的表达及其端粒酶活性的影响。结果显示,与对照组相比,各牛磺酸组MD肿瘤细胞chTERT转录水平和端粒酶活性均降低,其中1%、2%牛磺酸浓度组的chTERT转录水平和端粒酶活性显著低于对照组(P<0.05)。牛磺酸对chTERT转录水平及端粒酶活性的下调,可能抑制肿瘤细胞的增殖。
In order to explore effect of taurine on proliferation of MDCC-MSB1 cell and its possible mechanism,In proper concentration range of taurine, the MDCC-MSB1 cell were cultured in RPMI1640 medium with different concentration taurine which is respectively 0%,0.5%,1.0% and 2.0%. Telomerase activity and expression of and chTERT subunit were tested by semi-quantitative reverse transcriptase-polymerase chain reaction(RT-PCR) and TRAP techniques for its effect on the tumor cells.The results showed that telomerase activity and chTERT expression in mRNA level of taurine group cells are down regulated compared with the control group. Telomerase activity and the chTERT expression of 1%, 2% taurine groups are reduced significantly compared with the control group(P< 0.05). Taurine might inhibit proliferation of tumor cell by down regulating telomerase activity and chTERT expression in mRNA level.
In order to explore effect of taurine on proliferation of MDCC-MSB1 cell and its possible mechanism,In proper concentration range of taurine, the MDCC-MSB1 cell were cultured in RPMI1640 medium with different concentration taurine which is respectively 0%,0.5%,1.0% and 2.0%. Telomerase activity and expression of and chTERT subunit were tested by semi-quantitative reverse transcriptase-polymerase chain reaction(RT-PCR) and TRAP techniques for its effect on the tumor cells.The results showed that telomerase activity and chTERT expression in mRNA level of taurine group cells are down regulated compared with the control group. Telomerase activity and the chTERT expression of 1%, 2% taurine groups are reduced significantly compared with the control group(P< 0.05). Taurine might inhibit proliferation of tumor cell by down regulating telomerase activity and chTERT expression in mRNA level.
引文
[1]张婷,王晓民.端粒、端粒酶的发现与意义[J].首都医科大学学报.2009,30(5):718-721.
[2]吴东林.端粒重复扩增程序(TRAP)的改进和应用以及端粒酶酶学性质研究[D].长春:解放军军需大学,2001.
[3]邵晋晨,吴继锋.端粒酶与细胞周期及细胞凋亡关系的研究进展[J].国外医学,2002,22(1):36-39.
[4]Dumoulin J C,Evers J L,Bakker J A,et al.Temporal effects of taurine on mouse preimplantation development in vitro[J].J.Assist Reprod Genet.,1992,9(1):68-76.
[5]Oh B K,Kim H,Park Y N et al.High telomerase activity and long telomeres in advanced hepatocellular carcinomas with poor prognosis[J].Laboratory Investigation,2008,88:144-152.
[6]刘宇,骞宪肿,等.牛磺酸对肝癌大鼠肝细胞保护机制的研究.世界中西医结合杂志,2009,4(3):166-168.
[7]Dasika G K,Lin S C,Zhao S,et al.DNA damage-induced cell cycle checkpoints and DNA strand break repair in development and tumorigenesis[J].Oncogene,1999,18:7883-7899.
[8]盛方军.ATM对AT细胞端粒酶活性、hTERT表达影响研究[D].2006.
[2]吴东林.端粒重复扩增程序(TRAP)的改进和应用以及端粒酶酶学性质研究[D].长春:解放军军需大学,2001.
[3]邵晋晨,吴继锋.端粒酶与细胞周期及细胞凋亡关系的研究进展[J].国外医学,2002,22(1):36-39.
[4]Dumoulin J C,Evers J L,Bakker J A,et al.Temporal effects of taurine on mouse preimplantation development in vitro[J].J.Assist Reprod Genet.,1992,9(1):68-76.
[5]Oh B K,Kim H,Park Y N et al.High telomerase activity and long telomeres in advanced hepatocellular carcinomas with poor prognosis[J].Laboratory Investigation,2008,88:144-152.
[6]刘宇,骞宪肿,等.牛磺酸对肝癌大鼠肝细胞保护机制的研究.世界中西医结合杂志,2009,4(3):166-168.
[7]Dasika G K,Lin S C,Zhao S,et al.DNA damage-induced cell cycle checkpoints and DNA strand break repair in development and tumorigenesis[J].Oncogene,1999,18:7883-7899.
[8]盛方军.ATM对AT细胞端粒酶活性、hTERT表达影响研究[D].2006.