自噬相关蛋白LC3与Raptor、Rictor在甲状腺乳头状癌中的表达及临床意义
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摘要
目的观察自噬相关蛋白LC3、Raptor、Rictor在甲状腺乳头状癌中表达情况,分析其与临床病理因素之间的关系。方法采用免疫组织化学法检测LC3、Raptor、Rictor在甲状腺乳头状癌及其癌旁组织中的表达情况,并结合临床病理因素进行分析。结果 LC3、Raptor、Rictor在甲状腺乳头状癌中的阳性表达率分别为76. 7、82. 5、41. 7%,在癌旁组织中的阳性表达率分别为4. 9、16. 5、7. 8%,三项指标的表达差异均有统计学意义。LC3的表达与肿瘤长径及砂砾体形成相关,Raptor、Rictor的表达均与TNM分期相关,Rictor的表达与淋巴结转移相关。结论 LC3、Raptor、Rictor在甲状腺乳头状癌中表达升高,对于甲状腺乳头状癌的诊断、判断预后方面具有潜在价值。
Objective To investigate the expression of LC3,Raptor and Rictor in papillary thyroid carcinoma and elucidate the relationship between LC3,Raptor,Rictor and cancer clinical pathological factors. Methods Immunohistochemical method was used to detect LC3,Raptor and Rictor expression. The relationship with expression of LC3,Raptor,Rictor and clinicopathological factors was analyzed. Results The positive rates of LC3,Raptor and Rictor in papillary thyroid carcinoma were 76. 7,82. 5 and 41. 7% respectively,but they were 4. 9,16. 5 and 7. 8%,respectively in the adjacent tissues. The difference was statistically significant. LC3 was correlated with tumor size and psammoma bodies,High expressions of Raptor and Rictor were significantly correlated with TNM grade,and Rictor was correlated with lymph node metastasis. Conclusion The expression of LC3,Raptor and Rictor were up-regulated in papillary thyroid carcinoma,and they were considered to have potential value for the diagnosis and prognosis of papillary thyroid carcinoma.
Objective To investigate the expression of LC3,Raptor and Rictor in papillary thyroid carcinoma and elucidate the relationship between LC3,Raptor,Rictor and cancer clinical pathological factors. Methods Immunohistochemical method was used to detect LC3,Raptor and Rictor expression. The relationship with expression of LC3,Raptor,Rictor and clinicopathological factors was analyzed. Results The positive rates of LC3,Raptor and Rictor in papillary thyroid carcinoma were 76. 7,82. 5 and 41. 7% respectively,but they were 4. 9,16. 5 and 7. 8%,respectively in the adjacent tissues. The difference was statistically significant. LC3 was correlated with tumor size and psammoma bodies,High expressions of Raptor and Rictor were significantly correlated with TNM grade,and Rictor was correlated with lymph node metastasis. Conclusion The expression of LC3,Raptor and Rictor were up-regulated in papillary thyroid carcinoma,and they were considered to have potential value for the diagnosis and prognosis of papillary thyroid carcinoma.
引文
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[3]Smithson L J. Proteomic analysis reveals GIT1 as a novel m TOR complex component critical for mediating astrocyte survival[J]. Genes&development,2016,30(12):1383-1388.
[4]Li Z,Xin J,Chen W,et al. Genetic variants in autophagy associated genes are associated with DNA damage levels in Chinese population[J]. Gene,2017,626:414-419.
[5]Wu S,Sun C,Tian D,et al. Expression and clinical significances of Beclin1,LC3 and m TOR in colorectal cancer[J]. Int J Clin Exp Pathol,2015,8(4):3882-3891.
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[7]Sabaretnam M,Timothy J,Kamalaretnam M,et al. The prognostic relevance of psammoma bodies and ultrasonographic intratumoral calcifications in papillary thyroid carcinoma[J]. World journal of surgery,2014,38(3):748.
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[9]Efeyan A,Zoncu R,Chang S,et al. Regulation of m TORC1 by the Rag GTPases is necessary for neonatal autophagy and survival[J].Nature,2013,493(7434):679-683.
[10]Yang S,Xiao X,Meng X. A mechanism for synergy with combined m TOR and PI3 kinase inhibitors[J]. Plo S one,2011,6(10):e26343.
[11]Kalim K W,Zhang S,Chen X,et al. m TOR has a developmental stage-specific role in mitochondrial fitness independent of conventional m TORC1 and m TORC2 and the kinase activity[J]. Plo S one,2017,12(8):e0183266.
[12] Kang H G,Yoo S S,Choi J E,et al. Polymorphisms in mitotic checkpoint-related genes can influence survival outcomes of earlystage non-small cell lung cancer[J]. Oncotarget,2017,8(37):61777-61785.
[13]Wazir U,Newbold R F,Jiang W G,et al. Prognostic and therapeutic implications of m TORC1 and Rictor expression in human breast cancer[J]. Oncology reports,2013,29(5):1969-1974.
[14]Mikami S,Oya M,Kosaka T,et al. Increased vasohibin-1 expression is associated with metastasis and poor prognosis of renal cell carcinoma patients[J]. Laboratory investigation; a journal of technical methods and pathology,2017,97(7):854-862.
[15] Shuhua W,Chenbo S,Yangyang L,et al. Autophagy-related genes Raptor,Rictor,and Beclin1 expression and relationship with multidrug resistance in colorectal carcinoma[J〗. Human pathology,2015,46(11):1752-1759.