血浆血管内皮生长因子水平与无排卵性功血的相关性分析
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摘要
目的:探讨血浆血管内皮生长因子(VEGF)水平与无排卵性功血(ADUB)患者的子宫内膜微血管密度(MVD)的相关性。方法:以2018年1月至12月我院收治的ADUB患者100例为观察组,选择同期在我院进行体检的健康人员100例为对照组。分别采用酶联免疫(ELISA法)及免疫组织化学法对两组血浆VEGF水平与MVD计数进行检测,并予以相关性分析。结果:观察组和对照组血浆VEGF分别是10.68±1.12 pg/m L、9.83±1.07 pg/m L,P=0.000;观察组和对照组血浆MVD分别是24.85±2.85个/×100HP、17.44±2.39个/×100HP,P=0.000。Pearson相关性分析可得:ADUB患者血浆VEGF水平与子宫内膜MVD水平相关性P=0.000,R=0.62。结论:1.VEGF水平在ADUB患者的血浆呈现上升趋势。2.ADUB患者的血浆VEGF水平与子宫内膜MVD水平存在正相关性。
Objective: To determine the correlation between plasma vascular endothelial growth factor( VEGF) level and endometrial microvascular density( MVD) in patients with anovulatory dysfunctional uterine bleeding( ADUB). Methods: A total of 100 ADUB patients admitted to our hospital between January 2018 and December 2018 were included as the study group. A contemporary cohort of 100 healthy subjects who underwent physical examination in our hospital was included as the control group. Enzyme linked immunosorbent assay( ELISA) and immunohistochemistry were used to examine the plasma VEGF level and MVD counts for correlation analysis. Results: The plasma VEGF level in the study group and control group was 10.68±1.12 pg/m L and 9.83±1.07 pg/m L,respectively,P = 0.000. The plasma MVD counts in the study group and control group were 24.85+2.85/×100 HP and 17.44+2.39/×100 HP,respectively( P = 0.000). Pearson correlation analysis showed that a positive correlation between plasma VEGF level and endometrial MVD in ADUB patients( r = 0.62,P = 0.000).Conclusion: 1. The VEGF level in plasma of ADUB patients shows an increasing trend. 2. There is a positive correlation between plasma VEGF level and endometrial MVD in ADUB patients.
Objective: To determine the correlation between plasma vascular endothelial growth factor( VEGF) level and endometrial microvascular density( MVD) in patients with anovulatory dysfunctional uterine bleeding( ADUB). Methods: A total of 100 ADUB patients admitted to our hospital between January 2018 and December 2018 were included as the study group. A contemporary cohort of 100 healthy subjects who underwent physical examination in our hospital was included as the control group. Enzyme linked immunosorbent assay( ELISA) and immunohistochemistry were used to examine the plasma VEGF level and MVD counts for correlation analysis. Results: The plasma VEGF level in the study group and control group was 10.68±1.12 pg/m L and 9.83±1.07 pg/m L,respectively,P = 0.000. The plasma MVD counts in the study group and control group were 24.85+2.85/×100 HP and 17.44+2.39/×100 HP,respectively( P = 0.000). Pearson correlation analysis showed that a positive correlation between plasma VEGF level and endometrial MVD in ADUB patients( r = 0.62,P = 0.000).Conclusion: 1. The VEGF level in plasma of ADUB patients shows an increasing trend. 2. There is a positive correlation between plasma VEGF level and endometrial MVD in ADUB patients.
引文
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[15]Zygon J,Szajewski M,Kruszewski WJ,et al. Vegf,flt-1,and microvessel density in primary tumors as predictive factors of colorectal cancer prognosis[J]. Molecular and clinical oncology. 2017,6(2):243-248.
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[18]Yoshida K,Suzuki S,Sakata J,et al. The upregulated expression of vascular endothelial growth factor in surgically treated patients with recurrent/radioresistant cervical cancer of the uterus[J]. Oncology letters. 2018,16(1):515-521.
[2]Zinovkin DA,Pranjol MZI,Petrenyov DR,et al. The potential roles of melf-pattern,microvessel density,and vegf expression in survival of patients with endometrioid endometrial carcinoma:A morphometrical and immunohistochemical analysis of 100 cases[J]. Journal of pathology and translational medicine. 2017,51(5):456-462.
[3] Liu HZ,Han XX,Liu J,et al. Effect of taoren quyu decoction on human endometrial cells and its antiendometriosis activity in rats[J]. Asian Pacific journal of tropical medicine. 2017,10(7):696-700.
[4] Chen HX,Xu XX,Tan BZ,et al. Microrna-29b inhibits angiogenesis by targeting vegfa through the mapk/erk and pi3k/akt signaling pathways in endometrial carcinoma[J].Cellular physiology and biochemistry:international journal of experimental cellular physiology, biochemistry, and pharmacology. 2017,41(3):933-946.
[5] Guan J,Darb-Esfahani S,Richter R,et al. Vascular endothelial growth factor receptor 2(vegfr2)correlates with long-term survival in patients with advanced highgrade serous ovarian cancer(hgsoc):A study from the tumor bank ovarian cancer(toc)consortium[J]. Journal of cancer research and clinical oncology. 2019.
[6] Hirose M,Matsumuro A,Arai T,et al. Serum vascular endothelial growth factor-d as a diagnostic and therapeutic biomarker for lymphangioleiomyomatosis[J]. PloS one.2019,14(2):e0212776.
[7] Huang YW,Tsai HC,Wang SW,et al. Amphiregulin promotes vascular endothelial growth factor-c expression and lymphangiogenesis through stat3 activation in human chondrosarcoma cells[J]. Cellular physiology and biochemistry:international journal of experimental cellular physiology, biochemistry, and pharmacology. 2019,52(1):1-15.
[8] Schachat AP. If there is loss to follow up,poor outcomes may be more common after anti-vascular endothelial growth factor treatment for diabetic retinopathy[J].Ophthalmology. 2019,126(3):414.
[9]Semrad TJ,Groshen S,Luo C,et al. Randomized phase 2study of trebananib(amg 386)with or without continued anti-vascular endothelial growth factor therapy in patients with renal cell carcinoma who have progressed on bevacizumab,pazopanib,sorafenib,or sunitinib-results of nci/ctep protocol 9048[J]. Kidney cancer. 2019,3(1):51-61.
[10]Elfayomy AK,Almasry SM,Attia GM,et al. Enhanced expression of vascular endothelial growth factor and increased microvascular density in women with endometrial hyperplasia:A possible relationship with uterine natural killer cells[J]. Romanian journal of morphology and embryology=Revue roumaine de morphologie et embryologie. 2015,56(2 Suppl):725-734.
[11]Yeh CC,Shih LJ,Chang JL,et al. Synchronous vascular endothelial growth factor protein profiles in both tissue and serum identify metastasis and poor survival in colorectal cancer[J]. Scientific reports. 2019,9(1):4228.
[12]Sanchis EG,Cristofolini AL,Merkis CI. Porcine placental immunoexpression of vascular endothelial growth factor,placenta growth factor,flt-1 and flk-1[J]. Biotechnic&histochemistry:official publication of the Biological Stain Commission. 2015,90(7):486-494.
[13] Xiao Z,Li S,Yu Y,et al. Vegf-a regulates sflt-1production in trophoblasts through both flt-1 and kdr receptors[J]. Molecular and cellular biochemistry. 2018,449(1-2):1-8.
[14] Gray RT,O'Donnell ME,Maxwell P,et al. Long-term follow-up of immunocytochemical analysis of vascular endothelial growth factor(vegf),and its two receptors,vegf-r1(flt-1)and vegf-r2(flk-1/kdr), in oesophagogastric cancer[J]. The International journal of biological markers. 2013,28(1):63-70.
[15]Zygon J,Szajewski M,Kruszewski WJ,et al. Vegf,flt-1,and microvessel density in primary tumors as predictive factors of colorectal cancer prognosis[J]. Molecular and clinical oncology. 2017,6(2):243-248.
[16] Sagsoz H,Liman N,Alan E. Expression of vascular endothelial growth factor receptors and their ligands in rat uterus during the postpartum involution period[J].Biotechnic&histochemistry:official publication of the Biological Stain Commission. 2015,90(5):361-374.
[17]Agaoglu OK,Agaoglu AR,Guzeloglu A,et al. Expression of hypoxia-inducible factors and vascular endothelial growth factor during pregnancy in the feline uterus[J].Theriogenology. 2015,84(1):24-33.
[18]Yoshida K,Suzuki S,Sakata J,et al. The upregulated expression of vascular endothelial growth factor in surgically treated patients with recurrent/radioresistant cervical cancer of the uterus[J]. Oncology letters. 2018,16(1):515-521.