Th1/Th2/Th17/Treg平衡在特应性皮炎中的变化规律
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摘要
目的探讨Th1/Th2/Th17/Treg平衡在特应性皮炎中的变化规律。方法收集2014年2月-2017年1月在本院收治的56例确诊的特应性皮炎患儿,其中轻症11例、中症29例和重症16例。选择同期的健康儿童26例为对照组。检测外周血中IFN-y、IL-4、IL-17、IL-21、IL-23、IL-10和转化生长因子(TGF-")水平。结果与对照组相比,特应性皮炎组的IL-4、IL-17、IL-21、IL-23均明显增高,差异有统计学意义(P<0.05),且呈严重程度依赖性增高,其余指标IFN-γ有降低趋势,IL-10和TGF-"有增高趋势,且呈严重程度依赖性变化,但差异无统计学意义(P>0.05)。与对照组相比,特应性皮炎组的IFN-y/IL-4、IFN-y/IL-17、IFN-y/IL-21、IFN-y/IL-23、IL-10/IL-17和TGF-"/IL-17均明显降低,差异有统计学意义(P<0.05),且呈严重程度依赖性降低。结论 Th1、Th2、Th17和Treg细胞均参与了特应性皮炎的发生发展过程,Th2和Th17细胞处于主导地位。
Objective To investigate the Th1/Th2/Th17/Treg balance in atopic dermatitis.Methods A total of 56 patients with atopic dermatitis admitted to our hospital from February 2014 to January 2017 were enrolled into the study. The number of patients eligible with mild,moderate and severe atopic dermatitis were 11,29 and 16,respectively. A total of 26 healthy children were enrolled as the control group. Interferon y( IFN-y),interleukin-4( IL-4),interleukin-17( IL-17),interleukin-23( interleukin-23),interleukin-10( IL-10),and transforming growth factor beta( TGF-β)in the blood were detected and compared. Results Compared with the control group,the IL-4,L-17,IL-21 and IL-23 in atopic dermatitis group were significantly increased in a severity-dependent manner( P < 0. 05). IFN-y was decreased,whereas both IL-10 and TGF-β" were up-regulated in a severity-dependent manner.However,there was no significant difference( P > 0. 05). IFN-y/IL-17,IFN-y/IL-21,IFN-y/IL-23,IL-10/IL-17,IFN-y/IL-17 and IFN-y/IL-17 were significantly decreased in a severity-dependent manner,suggesting a significant difference( P <0. 05). Conclusion Th1,Th2,Th17 and Treg cells are closely associated with thedevelopment of atopic dermatitis,and Th2 and Th17 are dominantly involved.
Objective To investigate the Th1/Th2/Th17/Treg balance in atopic dermatitis.Methods A total of 56 patients with atopic dermatitis admitted to our hospital from February 2014 to January 2017 were enrolled into the study. The number of patients eligible with mild,moderate and severe atopic dermatitis were 11,29 and 16,respectively. A total of 26 healthy children were enrolled as the control group. Interferon y( IFN-y),interleukin-4( IL-4),interleukin-17( IL-17),interleukin-23( interleukin-23),interleukin-10( IL-10),and transforming growth factor beta( TGF-β)in the blood were detected and compared. Results Compared with the control group,the IL-4,L-17,IL-21 and IL-23 in atopic dermatitis group were significantly increased in a severity-dependent manner( P < 0. 05). IFN-y was decreased,whereas both IL-10 and TGF-β" were up-regulated in a severity-dependent manner.However,there was no significant difference( P > 0. 05). IFN-y/IL-17,IFN-y/IL-21,IFN-y/IL-23,IL-10/IL-17,IFN-y/IL-17 and IFN-y/IL-17 were significantly decreased in a severity-dependent manner,suggesting a significant difference( P <0. 05). Conclusion Th1,Th2,Th17 and Treg cells are closely associated with thedevelopment of atopic dermatitis,and Th2 and Th17 are dominantly involved.
引文
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[7]Valero MA,Perez-Crespo I,Chillón-Marinas C,et al.Fasciola hepatica reinfection potentiates a mixed Th1/Th2/Th17/Treg response and correlates with the clinical phenotypes of anemia[J].PLo S One,2017,12(3):e0173456.
[8]Gharesi-Fard B,Mobasher-Nejad F,Nasri F.The expression of T-helper associated transcription factors and cytokine genes in pre-eclampsia[J].Iran J Immunol,2016,13(4):296-308.
[9]Ahmad SF,Zoheir KMA,Ansari MA,et al.Dysregulation of Th1,Th2,Th17,and T regulatory cell-related transcription factor signaling in children with autism[J].Mol Neurobiol,2017,54(6):4390-4400.
[10]Talaat RM,Mohamed SF,Bassyouni IH,et al.Th1/Th2/Th17/Treg cytokine imbalance in systemic lupus erythematosus(SLE)patients:Correlation with disease activity[J].Cytokine,2015,72(2):146-153.
[11]Wei P,Hu GH,Kang HY,et al.An aryl hydrocarbon receptor ligand acts on dendritic cells and T cells to suppress the Th17 response in allergic rhinitis patients[J].Lab Invest,2014,94(5):528-535.
[12]Jiang N,Liao W,Kuang X.Effects of emodin on IL-23/IL-17 inflammatory axis,Th17 cells and viral replication in mice with viral myocarditis[J].Nan Fang Yi Ke Da Xue Xue Bao,2014,34(3):373-378.
[13]Guggino G,Giardina AR,Raimondo S,et al.Targeting IL-6 signal-ling in early rheumatoidarthritis is followed by Th1and Th17 sup-pression and Th2 expansion[J].Clin Exp Rheumatol,2014,32(1):77-81.
[14]Lio PA,Lee K,Lakhani F.Response to"Case series study of the efficacy of compounded antibacterial,steroid,and moisturizer in atopic dermatitis"[J].Pediatr Dermatol,2017,34(6):745-746.
[2]Hlela C,Khumalo NP.Comment on"Prevention of flares in children with atopic dermatitis with regular use of an emollient containing glycerol and paraffin:A randomised controlled study"[J].Pediatr Dermatol,2017,34(6):743.
[3]Harris V,Fischer G.Comment on"Case Series Study of the Efficacy of Compounded Antibacterial,Steroid,and Moisturizer in Atopic Dermatitis"[J].Pediatr Dermatol,2017,34(6):744.
[4]Moosbrugger-Martinz V,Tripp CH,Clausen BE,et al.Atopic dermatitis induces the expansion of thymus-derived regulatory T cells exhibiting a Th2-like phenotype in mice[J].J Cell Mol Med,2016,20(5):930-938.
[5]Fu Y,Lou H,Wang C,et al.T cell subsets in cord blood are influenced by maternal allergy and associated with atopic dermatitis[J].Pediatr Allergy Immunol,2013,24(2):178-186.
[6]Hayashida S,Uchi H,Moroi Y,et al.Decrease in circulating Th17 cells correlates with increased levels of CCL17,Ig E and eosinophils in atopic dermatitis[J].J Dermatol Sci,2011,61(3):180-186.
[7]Valero MA,Perez-Crespo I,Chillón-Marinas C,et al.Fasciola hepatica reinfection potentiates a mixed Th1/Th2/Th17/Treg response and correlates with the clinical phenotypes of anemia[J].PLo S One,2017,12(3):e0173456.
[8]Gharesi-Fard B,Mobasher-Nejad F,Nasri F.The expression of T-helper associated transcription factors and cytokine genes in pre-eclampsia[J].Iran J Immunol,2016,13(4):296-308.
[9]Ahmad SF,Zoheir KMA,Ansari MA,et al.Dysregulation of Th1,Th2,Th17,and T regulatory cell-related transcription factor signaling in children with autism[J].Mol Neurobiol,2017,54(6):4390-4400.
[10]Talaat RM,Mohamed SF,Bassyouni IH,et al.Th1/Th2/Th17/Treg cytokine imbalance in systemic lupus erythematosus(SLE)patients:Correlation with disease activity[J].Cytokine,2015,72(2):146-153.
[11]Wei P,Hu GH,Kang HY,et al.An aryl hydrocarbon receptor ligand acts on dendritic cells and T cells to suppress the Th17 response in allergic rhinitis patients[J].Lab Invest,2014,94(5):528-535.
[12]Jiang N,Liao W,Kuang X.Effects of emodin on IL-23/IL-17 inflammatory axis,Th17 cells and viral replication in mice with viral myocarditis[J].Nan Fang Yi Ke Da Xue Xue Bao,2014,34(3):373-378.
[13]Guggino G,Giardina AR,Raimondo S,et al.Targeting IL-6 signal-ling in early rheumatoidarthritis is followed by Th1and Th17 sup-pression and Th2 expansion[J].Clin Exp Rheumatol,2014,32(1):77-81.
[14]Lio PA,Lee K,Lakhani F.Response to"Case series study of the efficacy of compounded antibacterial,steroid,and moisturizer in atopic dermatitis"[J].Pediatr Dermatol,2017,34(6):745-746.