搜索页_+Single+cell+cloning

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在“Elsevier电子期刊”中，命中：1,139条，耗时：0.1229405 秒

在所有数据库中总计命中：1,577条

1.Constitutive expression and characterization of a surface SRS (NcSRS67) protein of Neospora caninum with no orthologue in Toxoplasma gondii

作者：Marcos Alexandre Bezerra^a ; Luiz Miguel Pereira^a ; Aline Bononi^a ; Carla Agostino Biella^a ; Luciana Baroni^a ; Leticia Pollo-Oliveira^b ; Ana Patrí ; cia Yatsuda^a ; ^b ; ^{ayatsuda@fcfrp.usp.br}

关键词：Neospora caninum ; Apicomplexa ; Surface protein ; SRS proteins ; NcSRS67 ; Plasmid pCR-Blunt II-TOPO

刊名：Parasitology International

出版年：2017

2.Cell cloning-on-the-spot by using an attachable silicone cylinder

关键词：Cell cloning ; Single cell cloning ; Silicone-made cloning cylinder ; MDA-MB-231 breast cancer cell line

刊名：Biochemical and Biophysical Research Communications

出版年：2016

3.Rapid high-throughput cloning and stable expression of antibodies in HEK293 cells

作者：Jared L. Spidel ; ^{jspidel@morphotek.com} ; Benjamin Vaessen ; Yin Yin Chan ; Luigi Grasso ; J. Bradford Kline

关键词：Monoclonal antibody ; High-throughput cloning ; Bicistronic ; Transfection ; Stable cell line

刊名：Journal of Immunological Methods

出版年：2016

4.Refined protocol for generating monoclonal antibodies from single human and murine B cells

关键词：Monoclonal antibodies ; Single-cell PCR ; Human antibodies ; Gibson assembly ; Antibody expression

刊名：Journal of Immunological Methods

出版年：2016

5.T-cell libraries allow simple parallel generation of multiple peptide-specific human T-cell clones

作者：Sarah M. Theaker^a ; Cristina Rius^a ; Alexander Greenshields-Watson^a ; Angharad Lloyd^a ; Andrew Trimby^a ; Anna Fuller^a ; John J. Miles^a ; ^b ; David K. Cole^a ; Mark Peakman^c ; Andrew K. Sewell^a ; ¹ ; ^{sewellak@cf.ac.uk" class="auth_mail" title="E-mail the corresponding author} ; Garry Dolton^a ; ¹

关键词：APC ; Antigen presenting cells ; CDH3 ; cadherin-3 ; 51Cr ; chromium-51 ; DC ; dendritic cell ; EBOV-Z ; Zaire Ebola virus ; EBV ; Epstein&ndash ; Barr virus ; ELISA ; enzyme-linked immunosorbent assay ; ELISpot ; enzyme-linked immunospot assay ; EN2 ; Engrailed-2 ; flu ; influenza A ; FBS ; foetal bovine serum ; GAD65 ; glutamic acid decarboxylase ; gp ; glycoprotein ; HA ; haemagglutinin ; HLA ; human leukocyte antigen ; IGRP ; islet-specific glucose-6-phosphatase catalytic subunit-related protein ; IMP-3 ; Insulin-like growth

刊名：Journal of Immunological Methods

出版年：2016

6.Effects of Panax notoginseng saponins on proliferation and differentiation of rat embryonic cortical neural stem cells

作者：Yinchu Si^a ; Jun Zhu^b ; Xiang Huang^c ; Peichun Zhu^a ; Chune Xie^d ; ^{chunexie@126.com" class="auth_mail" title="E-mail the corresponding author}

关键词：basic fibroblast growth factor ; brain-derived neurotrophic factor ; cell culture ; embryonic rat ; neural stem cells ; Panax notoginseng saponins

刊名：Journal of the Chinese Medical Association

出版年：2016

7.A novel, rapid and efficient method of cloning functional antigen-specific T-cell receptors from single human and mouse T-cells

作者：Hiroshi Hamana ; Kiyomi Shitaoka ; Hiroyuki Kishi ; ^{immkishi@med.u-toyama.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Tatsuhiko Ozawa ; Atsushi Muraguchi

关键词：T-cell receptor ; Multiplex one-step RT-PCR ; Transcriptionally active PCR ; Luciferase reporter assay

刊名：Biochemical and Biophysical Research Communications

出版年：2016

8.ROCK inhibitor Y27632 promotes proliferation and diminishes apoptosis of marmoset induced pluripotent stem cells by suppressing expression and activity of caspase 3

作者：Yuehong Wu^a ; ^b ; Jianhong Shu^a ; Chengwen He^b ; Min Li^b ; Yujiong Wang^b ; Wenbin Ou^a ; Yulong He^a ; ^b ; ^{wuyh77@zstu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; ^{heyulong2003@163.com" class="auth_mail" title="E-mail the corresponding author}

关键词：Marmoset ; Induced pluripotent stem cell ; Rho-associated kinase inhibitor Y27632 ; Apoptosis ; Caspase 3

刊名：Theriogenology

出版年：2016

9.Localization microscopy of DNA in situ using Vybrant^® DyeCycleℿViolet fluorescent probe: A new approach to study nuclear nanostructure at single molecule resolution

作者：Dominika Żurek-Biesiada^a ; Aleksander T. Szczurek^b ; Kirti Prakash^b ; ^c ; Giriram K. Mohana^b ; Hyun-Keun Lee^b ; ^d ; Jean-Yves Roignant^b ; Udo J. Birk^b ; ^d ; Jurek W. Dobrucki^a ; ^{jerzy.dobrucki@uj.edu.pl" class="auth_mail" title="E-mail the corresponding author} ; Christoph Cremer^b ; ^c ; ^d ; ^{c.cremer@imb-mainz.de" class="auth_mail" title="E-mail the corresponding author}

关键词：40.040 ; Nuclear organization ; 70.060 ; Chromatin ; 160.010 ; Cellular imaging techniques ; 160.050 ; Light microscopy ; 20.050 ; DNA replication ; 110.030 ; DNA ; RNA and cloning

刊名：Experimental Cell Research

出版年：2016

10.Production of a soluble single-chain variable fragment antibody against okadaic acid and exploration of its specific binding

作者：Kuo He^a ; ^b ; Xiuyuan Zhang^b ; Lixia Wang^b ; Xinjun Du^a ; Dong Wei^b ; ^{weidongss1971@sina.com" class="auth_mail" title="E-mail the corresponding author}

关键词：Okadaic acid ; CLEIA ; Single-chain variable fragment ; Binding complex

刊名：Analytical Biochemistry

出版年：2016

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