搜索页_ Single-guide RNA (sgRNA)

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在“Elsevier电子期刊”中，命中：18条，耗时：0.0639715 秒

在所有数据库中总计命中：39条

1.In Silico Meets In Vivo: Towards Computational CRISPR-Based sgRNA Design

作者：Guo-hui Chuai¹ ; ³ ; Qi-Long Wang² ; ³ ; Qi Liu¹ ; ^{qiliu@tongji.edu.cn}

关键词：CRISPR ; in silico sgRNA design ; off-target ; genome editing

刊名：Trends in Biotechnology

出版年：2017

2.CRISPR interference-guided balancing of a biosynthetic mevalonate pathway increases terpenoid production

作者：Seong Keun Kim^a ; ^b ; Gui Hwan Han^a ; Wonjae Seong^a ; ^b ; Haseong Kim^a ; ^b ; Seon-Won Kim^c ; Dae-Hee Lee^a ; ^b ; ^{dhlee@kribb.re.kr" class="auth_mail" title="E-mail the corresponding author} ; Seung-Goo Lee^a ; ^b ; ^{sglee@kribb.re.kr" class="auth_mail" title="E-mail the corresponding author}

关键词：CRISPR ; Clustered regularly interspaced short palindromic repeats ; CRISPRi ; Clustered regularly interspaced short palindromic repeats interference ; MVA ; mevalonate ; sgRNA ; single guide RNA ; dCas9 ; inactive Cas9 ; IPP ; isopentenyl diphosphate ; DMAPP ; dimethylallyl diphosphate ; MEP ; methylerythritol 4-phosphate ; DXP ; 1-deoxy-D-xylulose 5-phosphate ; HMG-CoA ; 3-hydroxy-3-methyl-glutaryl-CoA ; LB ; Luria-Bertani ; TB ; terrific broth ; PCR ; polymerase chain reaction ; UTR ; untranslated region ; T7RNAP ; T7 RN

刊名：Metabolic Engineering

出版年：2016

3.A novel sgRNA selection system for CRISPR-Cas9 in mammalian cells

作者：Haiwei Zhang^a ; Xixi Zhang^a ; Cunxian Fan^a ; Qun Xie^a ; ^b ; Chengxian Xu^a ; Qun Zhao^a ; Yongbo Liu^a ; Xiaoxia Wu^a ; Haibing Zhang^a ; ^{hbzhang@sibs.ac.cn" class="auth_mail" title="E-mail the corresponding author}

关键词：CRISPR/Cas9 ; sgRNA selection ; Gene knockout ; Necroptosis ; Cell death

刊名：Biochemical and Biophysical Research Communications

出版年：2016

4.Pseudorabies virus can escape from CRISPR-Cas9-mediated inhibition

作者：Zhiyuan Peng¹ ; Ting Ouyang¹ ; Daxin Pang¹ ; Teng Ma ; Xinrong Chen ; Ning Guo ; Fuwang Chen ; Lin Yuan ; Hongsheng Ouyang ; Linzhu Ren ; ^{renlz@jlu.edu.cn" class="auth_mail" title="E-mail the corresponding author}

关键词：Pseudorabies virus (PRV) ; CRISPR/Cas9 ; Single-guide RNA (sgRNA) ; UL30 protein ; Inhibition ; Escape

刊名：Virus Research

出版年：2016

5.Tandem repeat knockout utilizing the CRISPR/Cas9 system in human cells

作者：Qingyan Lv¹ ; Liangxue Lai¹ ; Lin Yuan ; Yuning Song ; Tingting Sui ; Zhanjun Li ; ^{lizj_1998@jlu.edu.cn" class="auth_mail" title="E-mail the corresponding author}

关键词：sgRNA ; Single guide RNA ; Humanized Cas9 ; hCas9 ; Humanized S. pyogenes ; hSpCas9 ; Double-strand DNA breaks ; DSBs ; Tandem repeat units ; TRs ; Potential off-target sites located in the genome ; POTS ; Off-target sites located in the TRs ; OTS ; Protospacer adjacent motif ; PAM

刊名：Gene

出版年：2016

6.CRISPR/Cas9 knockout of HAS2 in rat chondrosarcoma chondrocytes demonstrates the requirement of hyaluronan for aggrecan retention

作者：Yi Huang ; Emily B. Askew ; Cheryl B. Knudson ; Warren Knudson ; ^{knudsonw@ecu.edu}

关键词：OA ; osteoarthritis ; ECM ; extracellular matrix ; HA ; hyaluronan ; HAS2 ; hyaluronan synthase-2 ; RCS ; rat chondrosarcoma ; sgRNA ; single guide RNA ; sGAG ; sulfated glycosaminoglycan ; DMMB ; dimethylmethylene blue

刊名：Matrix Biology

出版年：2016

7.Controlling transcription in human pluripotent stem cells using CRISPR-effectors

作者：Ryan M. Genga ; Nicola A. Kearns ; René ; Maehr ; ^{rene.maehr@umassmed.edu" class="auth_mail" title="E-mail the corresponding author}

关键词：CAG ; hybrid of cytomegalovirus immediate early enhancer ; chicken β-actin promoter and intron ; rabbit β-globin splice acceptor ; Cas ; CRISPR-associated ; CRISPR ; clustered regularly interspaced short palindromic repeats ; crRNA ; CRISPR-derived RNA ; dCas9 ; nuclease-dead Cas9 ; hPSC ; human pluripotent stem cell ; KRAB ; Krueppel repressor associated box ; mESC ; mouse embryonic stem cell ; PAM ; protospacer adjacent motif ; sgRNA ; single guide RNA ; Sp ; Streptococcus pyogenes ; tracrRNA ; trans-activating CRISPR

刊名：Methods

出版年：2016

8.Transcriptional induction of protein kinase C delta by p53 tumor suppressor in the apoptotic response to DNA damage

作者：Nurmaa Dashzeveg ; Satomi Yogosawa ; Kiyotsugu Yoshida ; ^{kyoshida@jikei.ac.jp" class="auth_mail" title="E-mail the corresponding author}

关键词：PKCδ ; protein kinase C delta ; ADR ; adriamycine ; BS ; binding site ; ns siRNA ; non-silencing siRNA ; CRISPRi ; clustered regularly interspaced short palindromic repeat interference ; dCAS9 ; dead Cas9 ; sgRNA ; single guide RNA ; wt-p53 / PKCδ ; wild type p53 / PKCδ ; mt-PKCδ / p53 ; mutant PKCδ / p53

刊名：Cancer Letters

出版年：2016

9.Advancements in zebrafish applications for 21st century toxicology

作者：Gloria R. Garcia ; Pamela D. Noyes ; Robert L. Tanguay ; ^{robert.tanguay@oregonstate.edu" class="auth_mail" title="E-mail the corresponding author}

关键词：CRISPR ; Clustered ; regularly interspaced ; short palindromic repeats ; Dpf ; Days post fertilization ; DSB ; Double strand break ; Hpf ; Hours post fertilization ; HSC ; Hematopoietic stem cell ; HTS ; High-throughput screen ; KO ; Knockout ; MO ; Morpholino oligonucleotide ; NSC ; Neural stem cell ; PGE2 ; 16 ; 16-dimethyl prostaglandin E2 ; PMR ; Photomotor response ; RNAi ; RNA interference ; sgRNA ; Single guide RNA

刊名：Pharmacology and Therapeutics

出版年：2016

10.CRISPR/Cas9 genome editing of rubber producing dandelion Taraxacum kok-saghyz using Agrobacterium rhizogenes without selection

作者：Brian Iaffaldano ; Yingxiao Zhang ; Katrina Cornish ; ^{cornish.19@osu.edu" class="auth_mail" title="E-mail the corresponding author}

关键词：TK ; Taraxacum kok-saghyz ; 1-FFT ; fructan ; fructan 1-fructosyltransferase ; ZFNs ; zinc finger nucleases ; TALENs ; transcription activator-like effector nucleases ; CRISPR ; clustered regularly interspaced short palindromic repeats ; Cas9 ; CRISPR associated protein 9 ; QTLs ; quantitative trait loci ; PEG ; polyethylene glycol ; 1/2 MS ; half-strength Murashige and Skoog medium ; sgRNA ; single guide RNA ; CTAB ; cetyl trimethyl ammonium bromide ; PCR ; polymerase chain reaction ; InDels ; insertions and deletions ; P

刊名：Industrial Crops & Products

出版年：2016

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