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在“Elsevier电子期刊”中，命中：1,055条，耗时：0.0239876 秒

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1.Quantitative multi-target RNA profiling in Epstein-Barr virus infected tumor cells

作者：A.E. Greijer¹ ; O. Ramayanti¹ ; S.A.W.M. Verkuijlen ; Z. Novalić ; H. Juwana ; J.M. Middeldorp ; ^{j.middeldorp@vumc.nl}

关键词：Epstein-Barr virus ; Quantitative RT-PCR ; RNA expression profile

刊名：Journal of Virological Methods

出版年：2017

2.Ct shift: A novel and accurate real-time PCR quantification model for direct comparison of different nucleic acid sequences and its application for transposon quantifications

作者：Orsolya Kolacsek ; Enikő Pergel ; Nó ; ra Varga ; Á ; gota Apá ; ti ; Tamá ; s I. Orbá ; n ; ^{orban.tamas@ttk.mta.hu}

关键词：qPCR ; quantitative polymerase chain reaction ; Ct ; threshold cycle ; ΔΔCt ; comparative Ct ; RT-PCR ; reverse transcription PCR ; PGBD3 ; PiggyBac derived transposon family member 3 ; CS ; Cockayne syndrome ; CSB ; Cockayne syndrome B ; SB ; Sleeping Beauty ; Rcor1 ; REST corepressor 1 ; EGFP ; enhanced green fluorescent protein ; IRDR-L ; inverted repeat direct repeat &ndash ; left ; IRDR-R ; inverted repeat direct repeat &ndash ; right ; plst ; plasmid standard ; RPPH1 ; Ribonuclease P RNA component H1 ; Hsp90 ; heat shoc

刊名：Gene

出版年：2017

3.Cigarette smoking represses expression of cytokine IL-12 and its regulator miR-21—An observational study in patients with coronary artery disease

作者：T.B. Opstad ; MSc ; PhD^a ; ^b ; ^c ; ^{trineoa@medisin.uio.no} ; B.S. Brusletto ; MSc^d ; H. Arnesen ; MD^a ; ^b ; ^c (Prof.) ; A.Å ; . Pettersen ; MD ; PhD^a ; ^b ; I. Seljeflot ; PhD^a ; ^b ; ^c (Prof.)

关键词：ath-159a ; Arabidopsis thaliana-159a ; ASCET ; Aspirin non-responsiveness and Clopidogrel Endpoint Trial ; CSE ; cigarette smoke extract ; Ct ; cycle threshold ; EDTA ; ethylenediaminetetraacetic acid ; HWE ; Hardy-Weinberg equilibrium ; let-7 ; Caenorhabditis elegans-7 ; miR ; micro-RNA ; PCR ; polymerase chain reaction ; PDCD4 ; Programmed Cell Death 4 ; snRNA ; small nuclear RNA ; RNU ; small nucleolar RNA ; RQ ; relative quantification ; Th ; T-helper cell ; UTR ; untranslated region

刊名：Immunobiology

出版年：2017

4.Variables influencing the efficiency and interpretation of reverse transcription quantitative PCR (RT-qPCR): An empirical study using Bacteriophage MS2

作者：Jaclyn A. Miranda ; Grieg F. Steward ; ^{grieg@hawaii.edu}

关键词：RT-qPCR ; reverse transcription real-time quantitative PCR ; RT ; reverse transcription ; rRNA ; ribosomal RNA ; mRNA ; messenger RNA ; cDNA ; complementary DNA ; s.d. ; standard deviation ; Cq ; quantitation cycle ; MMLV ; Moloney murine leukemia virus ; AMV ; avian myeloblastosis virus

刊名：Journal of Virological Methods

出版年：2017

5.Quantification of Pea enation mosaic virus 1 and 2 during infection of Pisum sativum by one step real-time RT-PCR

作者：Juliette Doumayrou^a ; ¹ ; ^{juliette.doumayrou@gmail.com} ; Melissa Sheber^a ; Bryony C. Bonning^b ; W. Allen Miller^a ;

关键词：Pea enation mosaic virus ; Pisum sativum ; Normalized quantification ; Virus accumulation

刊名：Journal of Virological Methods

出版年：2017

6.Development and evaluation of a novel RT-qPCR based test for the quantification of HER2 gene expression in breast cancer

作者：Hicham El Hadi^a ; ^b ; ^{h.elhadi@mascir.com} ; Imane Abdellaoui-Maane^a ; ^b ; ^{i.abdellaoui@mascir.com} ; Denise Kottwitz^a ; ^{denisekottwitz@web.de} ; Manal El Amrani^a ; ^{dahmani_manale@yahoo.fr} ; Nadia Bouchoutrouch^a ; ^{n.bouchoutrouch@mascir.com} ; Zineb Qmichou^a ; ^{z.qmichou@mascir.com} ; Mehdi Karkouri^c ; ^{mehdi.karkouri@menara.ma} ; Hicham ElAttar^d ; Hassan Errihani^e ; Pedro L Fernandez^f ; ^{plfernan@clinic.ub.es} ; Youssef Bakri^b ; ^{ybakri@gmail.com} ; Hassan Sefrioui^a ; ^{h.sefrioui@mascir.com} ; Abdeladim Moumen^a ; ^{a.moumen@mascir.com}

关键词：RT-qPCR ; reverse transcription quantitative reel time polymerase chain reaction ; HER2 ; human epidermal growth factor receptor 2 ; GAPDH ; glucophosphate dehydrogenase ; PPIA ; peptidylprolyl isomerase A ; RPL37a ; Ribosomal protein L37-alpha ; RPL30 ; Ribosomal protein L30 ; MRPL19 ; mitochondrial ribosomal protein L19 ; PUM1 ; pumilio RNA-binding family member 1 ; UBB ; ubiquitin B ; IHC ; immunohistochemistry ; FISH ; fluorescence in situ hybridization ; ECG ; endogenous control gene

刊名：Gene

出版年：2017

7.A practical qPCR approach to detect TERRA, the elusive telomeric repeat-containing RNA

作者：Marianna Feretzaki ; Joachim Lingner ; ^{joachim.lingner@epfl.ch}

关键词：Telomeres ; Subtelomeres ; TERRA ; Transcriptional regulation ; qRT-PCR

刊名：Methods

出版年：2017

8.Digital RT-PCR method for hepatitis A virus and norovirus quantification in soft berries

作者：Audrey Fraisse ; Coralie Coudray-Meunier ; Sandra Martin-Latil ; Catherine Hennechart-Collette ; Sabine Delannoy ; Patrick Fach ; Sylvie Perelle ; ^{sylvie.perelle@anses.fr}

关键词：Hepatitis A virus ; Norovirus ; Digital RT-PCR ; RT-qPCR ; Soft berries ; PCR inhibition

刊名：International Journal of Food Microbiology

出版年：2017

9.Methods for the physical characterization and quantification of extracellular vesicles in biological samples

作者：Dé ; borah L.M. Rupert^a ; Virginia Claudio^a ; ^b ; Cecilia Lä ; sser^c ; Marta Bally^a ; ^d ; ^{bally@chalmers.se}

关键词：AFM ; atomic force microscopy ; BCA ; bicinchoninic acid ; Cryo-TEM ; cryo-transmission electron microscopy ; DLS ; dynamic light scattering ; dn/dC ; refractive index increment with concentration ; ELISA ; enzyme-linked immunosorbent assays ; EM ; electron microscopy ; EV ; extracellular vesicle ; FF-TEM ; freeze-fractioning transmission electron microscopy ; FSC ; forward scatter ; LSPR ; localized surface plasmon resonance ; MALS ; multi-angle light scattering ; mRNA ; messenger RNA ; NTA ; nanoparticle tracking analys

刊名：Biochimica et Biophysica Acta (BBA) - General Subjects

出版年：2017

10.Assays for precise quantification of total (including short) and elongated HIV-1 transcripts

作者：Philipp Kaiser^a ; ^c ; Sunil K. Joshi^a ; Peggy Kim^a ; Peilin Li^a ; Hongbing Liu^b ; Andrew P. Rice^b ; Joseph K. Wong^a ; Steven A. Yukl^a ; ^{Steven.yukl@ucsf.edu}

关键词：HIV-1 ; Latency ; Transcription ; Elongation ; TAR ; Short or abortive transcript

刊名：Journal of Virological Methods

出版年：2017

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