搜索页_+Reverse+transcription+and+quantitative+RT-PCR

常用资源

电子图书

期刊论文

学位会议

外文资源

特色专题

内部出版物

Wiley电子期刊(23)	SpringerLink电子期刊(2706)	Elsevier电子期刊(6119)
Springer电子图书(2)	NATURE电子期刊(5)	ACS电子期刊(44)

在“Elsevier电子期刊”中，命中：6,119条，耗时：0.0849621 秒

在所有数据库中总计命中：8,899条

1.Variables influencing the efficiency and interpretation of reverse transcription quantitative PCR (RT-qPCR): An empirical study using Bacteriophage MS2

作者：Jaclyn A. Miranda ; Grieg F. Steward ; ^{grieg@hawaii.edu}

关键词：RT-qPCR ; reverse transcription real-time quantitative PCR ; RT ; reverse transcription ; rRNA ; ribosomal RNA ; mRNA ; messenger RNA ; cDNA ; complementary DNA ; s.d. ; standard deviation ; Cq ; quantitation cycle ; MMLV ; Moloney murine leukemia virus ; AMV ; avian myeloblastosis virus

刊名：Journal of Virological Methods

出版年：2017

2.Detection of Babesia microti parasites by highly sensitive 18S rRNA reverse transcription PCR

作者：Amelia E. Hanron^a ; Zachary P. Billman^a ; ^b ; Annette M. Seilie^a ; Ming Chang^a ; Sean C. Murphy^a ; ^b ; ^c ; ^{murphysc@uw.edu}

关键词：Babesia ; 18S rRNA ; Transfusion ; RT-PCR ; Diagnostics ; Clinical microbiology

刊名：Diagnostic Microbiology and Infectious Disease

出版年：2017

3.Identification and evaluation of reference genes for expression studies by RT-qPCR during embryonic development of the emerging model organism, Macrobrachium olfersii

作者：Michael L. Jaramillo^a ; ¹ ; Dib Ammar^a ; ^b ; ¹ ; Ruth L. Quispe^c ; Frank Guzman^d ; Rogerio Margis^e ; Evelise M. Nazari^a ; ² ; ^{evelise.nazari@ufsc.br} ; Yara M.R. Mü ; ller^a ; ²

关键词：RT-qPCR ; real-time reverse transcription quantitative polymerase chain reaction ; cDNA ; complementary DNA ; MIQE ; Minimum Information for Publication of Quantitative Real-Time PCR Experiments ; β-act ; β-actin ; GAPDH ; glyceraldehyde-3-phosphate dehydrogenase ; RpL8 ; ribosomal protein L8 ; RpS6 ; ribosomal protein S6 ; EF-1α ; elongation factor-1α ; AK ; arginine kinase ; M ; muscle ; HPT ; hepatopancreas ; CG ; cerebral ganglia ; Cq ; quantification cycle ; Ct ; threshold cycle ; E ; efficiency ; NFs ; normalization fac

刊名：Gene

出版年：2017

4.Development of a novel real-time RT-PCR assay to detect Seneca Valley virus-1 associated with emerging cases of vesicular disease in pigs

作者：Veronica L. Fowler^a ; Russell H. Ransburgh^b ; Elizabeth G. Poulsen^b ; Jemma Wadsworth^a ; Donald P. King^a ; Valerie Mioulet^a ; Nick J. Knowles^a ; Susanna Williamson^c ; Xuming Liu^b ; ^d ; Gary A. Anderson^b ; ^d ; Ying Fang^d ; Jianfa Bai^b ; ^d ; ^{jbai@vet.ksu.edu}

关键词：Seneca Valley virus ; Senecavirus A ; Swine ; Veterinary diagnostics ; rRT-PCR ; Real-time

刊名：Journal of Virological Methods

出版年：2017

5.Ct shift: A novel and accurate real-time PCR quantification model for direct comparison of different nucleic acid sequences and its application for transposon quantifications

作者：Orsolya Kolacsek ; Enikő Pergel ; Nó ; ra Varga ; Á ; gota Apá ; ti ; Tamá ; s I. Orbá ; n ; ^{orban.tamas@ttk.mta.hu}

关键词：qPCR ; quantitative polymerase chain reaction ; Ct ; threshold cycle ; ΔΔCt ; comparative Ct ; RT-PCR ; reverse transcription PCR ; PGBD3 ; PiggyBac derived transposon family member 3 ; CS ; Cockayne syndrome ; CSB ; Cockayne syndrome B ; SB ; Sleeping Beauty ; Rcor1 ; REST corepressor 1 ; EGFP ; enhanced green fluorescent protein ; IRDR-L ; inverted repeat direct repeat &ndash ; left ; IRDR-R ; inverted repeat direct repeat &ndash ; right ; plst ; plasmid standard ; RPPH1 ; Ribonuclease P RNA component H1 ; Hsp90 ; heat shoc

刊名：Gene

出版年：2017

6.ERα inhibited myocardin-induced differentiation in uterine fibroids

作者：Xing-Hua Liao^a ; ^b ; ¹ ; ^{xinghualiao@hotmail.com} ; Jun-Yan Li^d ; ¹ ; Xiu-Mei Dong^a ; ^f ; Xiuhong Wang^e ; Yuan Xiang^a ; Hui Li^a ; Cheng-Xi Yu^a ; Jia-Peng Li^a ; Bai-Yin Yuan^a ; Jun Zhou^a ; ^c ; ^{zhoujun@wust.edu.cn} ; Tong-Cun Zhang^a ; ^b ; ^{zhangtongcun@wust.edu.cn}

关键词：USMC ; uterine smooth muscle cells ; α-SMA ; alpha smooth muscle actin ; PBS ; phosphate-buffered saline ; H&E ; hematoxylin and eosin ; DAPI ; 4&prime ; 6&prime ; -diamidino-2-phenylindole ; RT-PCR ; reverse-transcription polymerase chain reaction ; qRT-PCR ; quantitative real-time RT-PCR ; ChIP ; chromatin immunoprecipitation ; SERMs ; selective estrogen receptor modulators ; ERE ; estrogen receptor binding element ; ER ; estrogen receptors

刊名：Experimental Cell Research

出版年：2017

7.Ryanodine receptor genes of the rice stem borer, Chilo suppressalis: Molecular cloning, alternative splicing and expression profiling

作者：Y.C. Peng^a ; ¹ ; C.W. Sheng^a ; ¹ ; John E. Casida^b ; C.Q. Zhao^a ; ^{zcq@njau.edu.cn} ; Z.J. Han^a ; ^{zjhan@njau.edu.cn}

关键词：ANOVA ; analysis of variance ; CaM ; calmodulin ; cDNAs ; complementary DNAs ; DMRT ; Duncan's Multiple Range Test ; DMSO ; dimethyl sulphoxide ; G3PDH ; glyceraldehyde 3-phosphate dehydrogenase ; GSPs ; gene specific primers ; MIR ; mannosyltransferase ; IP3R and RyR ; MW ; molecular weight ; ORF ; open reading frame ; pI ; isoelectric point ; qRT-PCR ; quantitative RT-PCR ; RACE ; rapid amplification of cDNA ends ; RIH ; RyR and IP3R homology ; RT-PCR ; reverse transcription-polymerase chain reaction ; RyR ; ryanodine recept

刊名：Pesticide Biochemistry and Physiology

出版年：2017

8.Spliced leader-based analyses reveal the effects of polycyclic aromatic hydrocarbons on gene expression in the copepod Pseudodiaptomus poplesia

作者：Yunyun Zhuang^a ; ¹ ; Feifei Yang^a ; ^b ; ¹ ; Donghui Xu^a ; ² ; Hongju Chen^a ; Huan Zhang^a ; ^c ; ^{huan.zhang@uconn.edu} ; ^{huan.zhang2@gmail.com} ; Guangxing Liu^a ; ^{gxliu@ouc.edu.cn}

关键词：1 ; 2 NAPH ; 1 ; 2-dimethylnaphthalene ; PAHs ; Polycyclic aromatic hydrocarbons ; RACE ; Rapid amplification of cDNA ends ; RT-qPCR ; reverse-transcription quantitative PCR ; SL ; Spliced leader

刊名：Aquatic Toxicology

出版年：2017

9.Transient receptor potential melastatin (TRPM) 8 is expressed in freshly isolated native human odontoblasts

作者：Kento Tazawa ; Hideharu Ikeda ; ^{hms-ikeda.endo@tmd.ac.jp} ; Nobuyuki Kawashima ; Takashi Okiji

关键词：DAPI ; 4&prime ; 6-diamidino-2-phenylindole ; DMP1 ; dentin matrix protein 1 ; DSPP ; dentin sialophosphoprotein ; GAPDH ; glyceraldehyde 3-phosphate dehydrogenase ; TRP ; transient receptor potential ; TRPA1 ; transient receptor potential ankyrin 1 ; TRPM8 ; transient receptor potential melastatin 8 ; PCR ; polymerase chain reaction ; qRT-PCR ; quantitative reverse transcription polymerase chain reaction ; RT-PCR ; reverse transcription polymerase chain reaction

刊名：Archives of Oral Biology

出版年：2017

10.Identification and functional analysis of pointed homologs in Bombyx mori

作者：Hiromitsu Tanaka ; ^{htanaka1@affrc.go.jp} ; Aki Sagisaka

关键词：B. mori ; Bombyx mori ; BmPNTs ; B. mori Pointeds ; AMP ; antimicrobial peptides ; Imd ; the immune deficiency ; shRNA ; short hairpin RNA ; PCR ; polymerase chain reaction ; RT-PCR ; reverse transcription-PCR ; qRT-PCR ; quantitative reverse transcriptase-PCR ; ORF ; open reading frame ; RACE ; rapid amplification of the cDNA ends ; RP ; ribosomal protein ; MAPK ; mitogen-activated protein kinase ; PGRP ; peptidoglycan recognition protein

刊名：Gene

出版年：2017

按检索点细分(6119)

题名(41)

关键词(1244)

文摘(4459)

地址：北京市海淀区学院路29号邮编：100083

电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700