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在“Elsevier电子期刊”中，命中：601条，耗时：小于0.01 秒

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1.In trans promoter activation by enhancers in transient transfection

作者：N.A. Smirnov ; S.B. Akopov ; D.A. Didych ; L.G. Nikolaev ; ^lev@ibch.ru ; ^{kinvel@gmail.com}

关键词：CMV ; cytomegalovirus ; HSV-tk ; herpes simplex virus thymidine kinase ; RLT ; random ligation template ; 3C ; chromosome conformation capture ; Ptk ; herpes simplex virus thymidine kinase promoter

刊名：Gene

出版年：2017

2.Tailor-made fibroblast-specific and antibiotic-free interleukin 12 plasmid for gene electrotransfer-mediated cancer immunotherapy

作者：Urska Kamensek^a ; ^{ukamensek@onko-i.si} ; Natasa Tesic^b ; ^{natasa.tesic@fvz.upr.si} ; Gregor Sersa^a ; ^{gsersa@onko-i.si} ; Spela Kos^a ; ^{skos@onko-i.si} ; Maja Cemazar^a ; ^b ; ^{mcemazar@onko-i.si}

关键词：IL-12 ; interleukin 12 ; GET ; gene electrotransfer ; CMV ; cytomegalovirus ; EMA ; European medicines agency ; ORT ; operator-repressor titration ; SVEC4&ndash ; 10 ; murine endothelial cells ; HUVEC ; human umbilical vein endothelial cells ; L929 ; murine fibroblasts ; LPB ; murine fibrosarcoma cells ; WI-38 ; human fibroblasts ; GFP ; green fluorescent protein ; EF-1α/HTLV ; hybrid elongation factor-1α promoter ; ELISA ; enzyme-linked immunosorbent assay ; qRT-PCR ; quantitative reverse transcription polymerase chain rea

刊名：Plasmid

出版年：2017

3.The fluorescent two-hybrid assay for live-cell profiling of androgen receptor modulators

作者：Jacqueline Bogner^a ; Kourosh Zolghadr^a ; Ian Hickson^b ; Tina Romer^a ; ^{t.romer@chromotek.com} ; Larisa Yurlova^a

关键词：AR ; androgen receptor protein ; BHK ; baby hamster kidney ; CMV IE ; immediate early promoter of cytomegalovirus ; CRPC ; castration resistant prostate cancer ; CYP17 ; 17α-hydroxy/17 ; 20-lyase ; DAPI ; 4&prime ; 6-diamidino-2-phenylindole ; DBD ; DNA-binding domain ; DHT ; 5α-dihydrotestosterone ; DMEM ; Dulbecco&rsquo ; s Modified Eagle Medium ; DNA ; deoxyribonucleic acid ; F2H ; fluorescent two-hybrid ; FDA ; Food and Drug Administration ; GFP ; green fluorescent protein ; HCA ; high-content analysis ; HEK ; human embryoni

刊名：The Journal of Steroid Biochemistry and Molecular Biology

出版年：2017

4.Enhancing heterologous protein expression and secretion in HEK293 cells by means of combination of CMV promoter and IFNα2 signal peptide

作者：Ramó ; n Romá ; n^a ; ^{ramon.roman@uab.cat" class="auth_mail" title="E-mail the corresponding author} ; Joan Miret^a ; Federica Scalia^b ; Antoni Casablancas^a ; Martí ; Lecina^a ; Jordi J. Cairó ; ^a

关键词：ALB ; human albumin ; CAG ; CMV early enhancer/chicken beta actin promoter ; CMV ; human Cytomegalovirus intermediate-early enhancer promoter ; EF-1α ; elongation factor 1 ; eGFP ; enhanced green fluorescent protein ; FBS ; fetal bovine serum ; GAU ; Gaussia princeps Luciferase ; HEK293 ; human embryonic kidney 293 cells ; IFNα2 ; Interferon alpha 2 ; IgG ; immunoglobulin G kappa chain ; IL-2 ; interleukin-2 ; IRES ; internal ribosome entry site ; NOSP ; no signal peptide ; SP ; signal peptide

刊名：Journal of Biotechnology

出版年：2016

5.Precision control of recombinant gene transcription for CHO cell synthetic biology

作者：Adam J. Brown ; David C. James ; ^{d.c.james@sheffield.ac.uk" class="auth_mail" title="E-mail the corresponding author}

关键词：CHO ; Chinese hamster ovary ; Cirp ; cold-inducible RNA-binding protein ; CMV-IE ; cytomegalovirus immediate early ; CRISPR ; clustered regularly interspaced short palindromic repeats ; DBD ; DNA binding domain ; DTE ; difficult-to-express ; EF1α ; elongation factor 1 alpha ; ER ; endoplasmic reticulum ; gRNA ; guide RNA ; hCMV-IE1 ; human cytomegalovirus immediate early 1 ; HT ; high-throughput ; LTR ; long terminal repeat ; MAb ; monoclonal antibody ; MAR ; matrix attachment region ; PIC ; pre-initiation complex ; SV40E ; s

刊名：Biotechnology Advances

出版年：2016

6.Analysis of the Fam181 gene family during mouse development reveals distinct strain-specific expression patterns, suggesting a role in nervous system development and function

作者：Matthias Marks^a ; ^b ; Tracie Pennimpede^a ; ¹ ; Lisette Lange^a ; ^b ; Phillip Grote^a ; ² ; Bernhard G. Herrmann^a ; ^c ; Lars Wittler^a ; ^{wittler@molgen.mpg.de" class="auth_mail" title="E-mail the corresponding author}

关键词：aa ; amino acid ; BAC ; bacterial artificial chromosome ; BLAST ; basic local alignment search tool ; bp ; base pairs ; cDNA ; complementary DNA ; cf ; compare figure ; c-hairy ; chick hairy homolog 1 ; CMV-Cre ; cytomegalovirus promoter driven Cre recombinase ; CO2 ; carbon dioxide ; CpG-methylation ; cytosine&ndash ; guanine dinucleotide-methylation ; DAPI ; 4&prime ; 6-diamidino-2-phenylindole ; DIG ; digoxigenin ; Dkk1 ; dickkopf homolog 1 ; Dll1 ; delta-like 1 ; DMEM ; Dulbecco's modified eagle medium ; DNA ; deoxyribonucle

刊名：Gene

出版年：2016

7.Controlling transcription in human pluripotent stem cells using CRISPR-effectors

作者：Ryan M. Genga ; Nicola A. Kearns ; René ; Maehr ; ^{rene.maehr@umassmed.edu" class="auth_mail" title="E-mail the corresponding author}

关键词：CAG ; hybrid of cytomegalovirus immediate early enhancer ; chicken β-actin promoter and intron ; rabbit β-globin splice acceptor ; Cas ; CRISPR-associated ; CRISPR ; clustered regularly interspaced short palindromic repeats ; crRNA ; CRISPR-derived RNA ; dCas9 ; nuclease-dead Cas9 ; hPSC ; human pluripotent stem cell ; KRAB ; Krueppel repressor associated box ; mESC ; mouse embryonic stem cell ; PAM ; protospacer adjacent motif ; sgRNA ; single guide RNA ; Sp ; Streptococcus pyogenes ; tracrRNA ; trans-activating CRISPR

刊名：Methods

出版年：2016

8.Development of an optimized cytotoxicity assay system for CYP3A4-mediated metabolic activation via modified piggyBac transposition

作者：Lizhen Huang^a ; ^c ; Shuxiang Zou^a ; Jifeng Deng^a ; Tianming Dai^a ; Jingwei Jiang^b ; Ying Jia^a ; Renke Dai^a ; Shuilin Xie^a ; ^c ; ^{hlzh2000518@163.com" class="auth_mail" title="E-mail the corresponding author}

关键词：CYP ; cytochrome P450 ; MTT ; 3-(4 ; 5-dimethyl-2-thiazolyl)-2 ; 5-diphenyl-2-H-tetrazolium bromide ; iPSCs ; induced pluripotent stem cells ; cDNA ; complementary DNA ; PB ; piggyBac ; PBase ; piggyBac tranposase ; BSO ; L-buthionine-(S ; R)-sulfoximine ; CMV ; cytomegalovirus promoter ; RFP ; red fluorescent protein ; PURO ; Puromycin ; DMEM ; Dulbecco's Modified Eagle Medium ; FCS ; fetal calf serum ; qRT-PCR ; quantitative real-time PCR ; GAPDH ; glyceraldehyde-3-phosphate dehydrogenase ; TBST ; Tris&ndash ; HCl buffered saline

刊名：Toxicology in Vitro

出版年：2016

9.Methylation-dependent and independent regulatory regions in the Na,K-ATPase alpha4 (Atp1a4) gene may impact its testis-specific expression

作者：Deepti L. Kumar¹ ; Priya L. Kumar¹ ; Paul F. James ; ^{jamespf@miamioh.edu" class="auth_mail" title="E-mail the corresponding author}

关键词：bp ; base pair ; CGI ; CpG island ; CMV ; human cytomegalovirus ; Dnmt ; DNA methyltransferase ; DMEM ; Dulbecco's Modified Eagle's media ; DKO ; double knockout ; ES cell ; mouse embryonic stem cell ; FBS ; fetal bovine serum ; HB-EGF-C ; C-terminal fragment of heparin-binding EGF-like growth factor ; HEK 293 ; human embryonic kidney 293 ; IACUC ; University Institutional Animal Care and Use Committee ; IV ; in vitro methylated ; kDa ; kilodalton ; KO ; knockout ; LIF ; leukemia inhibitory factor ; Mα4-CGI ; intragenic CpG i

刊名：Gene

出版年：2016

10.Sulfide exposure results in enhanced sqr transcription through upregulating the expression and activation of HSF1 in echiuran worm Urechis unicinctus

作者：Xiaolong Liu ; Zhifeng Zhang ; ^{zzfp107@ouc.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Xiaoyu Ma ; Xueyu Li ; Di Zhou ; Beibei Gao ; Yajiao Bai

关键词：SQR ; sulfidequinone oxidoreductase ; HSF1 ; heat shock factor 1 ; DBD ; DNA binding domain ; HR ; heptad repeats domain ; HSE ; heat shock element ; PSU ; practical salinity units ; RACE ; rapid amplification of cDNA ends ; IPTG ; isopropyl β-D-1-thiogalactopyranoside ; ChIP ; chromatin immunoprecipitation ; EMSA ; electrophoretic mobility shift assays ; BSA ; bovine serum albumin ; HRP ; streptavidin&ndash ; horseradish peroxidase ; PAGE ; polyacrylamide gel electrophoresis ; CMV ; cytomegalovirus ; ORF ; open reading frame

刊名：Aquatic Toxicology

出版年：2016

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