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在“Elsevier电子期刊”中，命中：1,091条，耗时：0.1899077 秒

在所有数据库中总计命中：1,907条

1.Variables influencing the efficiency and interpretation of reverse transcription quantitative PCR (RT-qPCR): An empirical study using Bacteriophage MS2

作者：Jaclyn A. Miranda ; Grieg F. Steward ; ^{grieg@hawaii.edu}

关键词：RT-qPCR ; reverse transcription real-time quantitative PCR ; RT ; reverse transcription ; rRNA ; ribosomal RNA ; mRNA ; messenger RNA ; cDNA ; complementary DNA ; s.d. ; standard deviation ; Cq ; quantitation cycle ; MMLV ; Moloney murine leukemia virus ; AMV ; avian myeloblastosis virus

刊名：Journal of Virological Methods

出版年：2017

2.Ct shift: A novel and accurate real-time PCR quantification model for direct comparison of different nucleic acid sequences and its application for transposon quantifications

作者：Orsolya Kolacsek ; Enikő Pergel ; Nó ; ra Varga ; Á ; gota Apá ; ti ; Tamá ; s I. Orbá ; n ; ^{orban.tamas@ttk.mta.hu}

关键词：qPCR ; quantitative polymerase chain reaction ; Ct ; threshold cycle ; ΔΔCt ; comparative Ct ; RT-PCR ; reverse transcription PCR ; PGBD3 ; PiggyBac derived transposon family member 3 ; CS ; Cockayne syndrome ; CSB ; Cockayne syndrome B ; SB ; Sleeping Beauty ; Rcor1 ; REST corepressor 1 ; EGFP ; enhanced green fluorescent protein ; IRDR-L ; inverted repeat direct repeat &ndash ; left ; IRDR-R ; inverted repeat direct repeat &ndash ; right ; plst ; plasmid standard ; RPPH1 ; Ribonuclease P RNA component H1 ; Hsp90 ; heat shoc

刊名：Gene

出版年：2017

3.MicroRNA-194 regulates keratinocyte proliferation and differentiation by targeting Grainyhead-like 2 in psoriasis

作者：Xiaoyun Yu^a ; ^b ; Jingang An^c ; Yunhui Hua^b ; Zihai Li^b ; Ning Yan^b ; Weixin Fan^d ; ^{fanweixin_njmu@163.com} ; Chuan Su^a ; ^{chuansu_njmu@163.com}

关键词：BrdU ; bromodeoxyuridine ; CK1 ; cytokeratin 1 ; CK10 ; cytokeratin 10 ; GRHL2 ; Grainyhead like 2 ; IL ; interleukin ; miRNAs ; microRNAs ; MTT ; 3-(4 ; 5-dimethyl-thiazol-2-yl)-2 ; 5-diphenyltetrazolium bromide ; RT-qPCR ; Real-time quantitative polymerase chain reaction ; STAT3 ; activation of signal transducer and activator of transcription-3 ; UTR ; 3&prime ; -untranslated region

刊名：Pathology - Research and Practice

出版年：2017

4.Identification and evaluation of reference genes for expression studies by RT-qPCR during embryonic development of the emerging model organism, Macrobrachium olfersii

作者：Michael L. Jaramillo^a ; ¹ ; Dib Ammar^a ; ^b ; ¹ ; Ruth L. Quispe^c ; Frank Guzman^d ; Rogerio Margis^e ; Evelise M. Nazari^a ; ² ; ^{evelise.nazari@ufsc.br} ; Yara M.R. Mü ; ller^a ; ²

关键词：RT-qPCR ; real-time reverse transcription quantitative polymerase chain reaction ; cDNA ; complementary DNA ; MIQE ; Minimum Information for Publication of Quantitative Real-Time PCR Experiments ; β-act ; β-actin ; GAPDH ; glyceraldehyde-3-phosphate dehydrogenase ; RpL8 ; ribosomal protein L8 ; RpS6 ; ribosomal protein S6 ; EF-1α ; elongation factor-1α ; AK ; arginine kinase ; M ; muscle ; HPT ; hepatopancreas ; CG ; cerebral ganglia ; Cq ; quantification cycle ; Ct ; threshold cycle ; E ; efficiency ; NFs ; normalization fac

刊名：Gene

出版年：2017

5.Quantification of Pea enation mosaic virus 1 and 2 during infection of Pisum sativum by one step real-time RT-PCR

作者：Juliette Doumayrou^a ; ¹ ; ^{juliette.doumayrou@gmail.com} ; Melissa Sheber^a ; Bryony C. Bonning^b ; W. Allen Miller^a ;

关键词：Pea enation mosaic virus ; Pisum sativum ; Normalized quantification ; Virus accumulation

刊名：Journal of Virological Methods

出版年：2017

6.ERα inhibited myocardin-induced differentiation in uterine fibroids

作者：Xing-Hua Liao^a ; ^b ; ¹ ; ^{xinghualiao@hotmail.com} ; Jun-Yan Li^d ; ¹ ; Xiu-Mei Dong^a ; ^f ; Xiuhong Wang^e ; Yuan Xiang^a ; Hui Li^a ; Cheng-Xi Yu^a ; Jia-Peng Li^a ; Bai-Yin Yuan^a ; Jun Zhou^a ; ^c ; ^{zhoujun@wust.edu.cn} ; Tong-Cun Zhang^a ; ^b ; ^{zhangtongcun@wust.edu.cn}

关键词：USMC ; uterine smooth muscle cells ; α-SMA ; alpha smooth muscle actin ; PBS ; phosphate-buffered saline ; H&E ; hematoxylin and eosin ; DAPI ; 4&prime ; 6&prime ; -diamidino-2-phenylindole ; RT-PCR ; reverse-transcription polymerase chain reaction ; qRT-PCR ; quantitative real-time RT-PCR ; ChIP ; chromatin immunoprecipitation ; SERMs ; selective estrogen receptor modulators ; ERE ; estrogen receptor binding element ; ER ; estrogen receptors

刊名：Experimental Cell Research

出版年：2017

7.Unexpected distribution of CA19.9 and other type 1 chain Lewis antigens in normal and cancer tissues of colon and pancreas: Importance of the detection method and role of glycosyltransferase regulation

作者：Adele Aronica^a ; Laura Avagliano^a ; Anna Caretti^a ; Delfina Tosi^a ; Gaetano Pietro Bulfamante^a ; ^b ; Marco Trinchera^c ; ^{marco.trinchera@uninsubria.it}

关键词：5AZA ; 5&rsquo ; aza-2&rsquo ; deoxycitidine ; B3GALT5 ; β1 ; 3 galactosyltransferase isoenzyme 5 ; ChIP ; chromatin immunoprecipitation ; DAPI ; 4 ; 6-diamidino-2- phenylindole ; FUT2 ; α1 ; 2 fucosyltransferase isoenzyme 2 ; FUT3 ; α1 ; 3/4 fucosyltransferase isoenzyme 3 ; H&E ; hematoxylin/eosin ; IF ; immunofluorescence ; IH ; immunohistochemistry ; Lea ; Lewis a ; Leb ; Lewis b ; LTR ; long terminal repeat ; qPCR ; quantitative real-time polymerase chain reaction ; RT ; reverse transcription ; sLea ; sialyl-Lewis a ; ST3GAL3 ; galac

刊名：Biochimica et Biophysica Acta (BBA) - General Subjects

出版年：2017

8.Foxc1 Ablated Mice Are Anhidrotic and Recapitulate Features of Human Miliaria Sweat Retention Disorder

作者：Chang-Yi Cui¹ ; ^{cuic@mail.nih.gov} ; Ryuga Ishii¹ ; Dean P. Campbell¹ ; Marc Michel¹ ; Yulan Piao¹ ; Tsutomu Kume² ; David Schlessinger¹

关键词：ChIP ; chromatin immunoprecipitation ; qRT-PCR ; quantitative real-time reverse transcriptase-PCR ; WT ; wild type

刊名：Journal of Investigative Dermatology

出版年：2017

9.Epigallocatechin-3-gallate inhibits adipogenesis through down-regulation of PPARγ and FAS expression mediated by PI3K-AKT signaling in 3T3-L1 cells

作者：Mengqing Wu^a ; ^b ; ¹ ; Dan Liu^a ; ^b ; ¹ ; Rong Zeng^a ; ^b ; ¹ ; Tao Xian^a ; ^b ; Yi Lu^a ; ^b ; Guohua Zeng^a ; ^b ; Zhangzetian Sun^c ; Bowei Huang^c ; Qiren Huang^a ; ^b ; ^{qrhuang@ncu.edu.cn}

关键词：4E-BP1 ; 4E-binding protein1 ; C/EBP ; CCAAT/ enhancer binding proteins ; EGCG ; epigallocatechin-3-gallate ; FAS ; fatty acid synthase ; FOXO1 ; forkhead box-O1 ; HFD ; high fat diet ; IGF ; insulin-like growth factor ; IR ; insulin resistance ; IRS1 ; insulin receptor substrate 1 ; MSCs ; mesenchymal stem cells ; PGC1 ; PPARγ coactivator 1 ; PI3K ; phosphatidylinositol 3 kinase ; PPARγ ; peroxisome proliferator activated receptor γ ; Rb ; retinoblastoma protein ; TG ; triglyceride ; TIF2 ; transcriptional intermediary facto

刊名：European Journal of Pharmacology

出版年：2017

10.Testicular gene expression of steroidogenesis-related factors in prepubertal, postpubertal, and aging dogs

作者：E. Ogawa ; N. Kawate ; T. Inaba ; H. Tamada ; ^{tamada@vet.osakafu-u.ac.jp}

关键词：Dog ; Testis ; Steroidogenesis ; Puberty ; Aging

刊名：Theriogenology

出版年：2017

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