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在“Elsevier电子期刊”中，命中：2,878条，耗时：0.0929563 秒

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1.Variables influencing the efficiency and interpretation of reverse transcription quantitative PCR (RT-qPCR): An empirical study using Bacteriophage MS2

作者：Jaclyn A. Miranda ; Grieg F. Steward ; ^{grieg@hawaii.edu}

关键词：RT-qPCR ; reverse transcription real-time quantitative PCR ; RT ; reverse transcription ; rRNA ; ribosomal RNA ; mRNA ; messenger RNA ; cDNA ; complementary DNA ; s.d. ; standard deviation ; Cq ; quantitation cycle ; MMLV ; Moloney murine leukemia virus ; AMV ; avian myeloblastosis virus

刊名：Journal of Virological Methods

出版年：2017

2.Characterization of the γ-aminobutyric acid signaling system in the zebrafish (Danio rerio Hamilton) central nervous system by reverse transcription-quantitative polymerase chain reaction

作者：Arianna Cocco ^{arianna.cocco@neuro.uu.se} ; A.M. Carolina Rö ; nnberg ^{carrr0-@hotmail.com} ; Zhe Jin ^{zhe.jin@neuro.uu.se} ; Gonç ; alo Igreja André ; ¹ ; ^{goncalo.igrejaandre@research.uwa.edu.au} ; Laura E. Vossen ^{laura.vossen@neuro.uu.se} ; Amol K. Bhandage ^{amol.bhandage@neuro.uu.se} ; Per-Ove Thö ; rnqvist ^{per-ove.thornqvist@neuro.uu.se} ; Bryndis Birnir ^{bryndis.birnir@neuro.uu.se} ; Svante Winberg ; ^{svante.winberg@neuro.uu.se}

关键词：abat ; 4-aminobutyrate aminotransferase ; actb1 ; actin β1 ; CCCs ; chloride-cation co-transporters ; EDTA ; ethylenediaminetetraacetic acid ; GABA ; γ-aminobutyric acid ; GABA-T ; GABA-α-ketoglutarate transaminase ; GAD ; glutamate decarboxylase ; RT-qPCR ; reverse transcription&ndash ; quantitative polymerase chain reaction ; SLC6 ; solute carrier 6 ; TAE ; Tris&ndash ; Acetate&ndash ; EDTA ; TBE ; Tris&ndash ; Borate&ndash ; EDTA ; tbp ; TATA-box binding protein ; tuba1b ; tubulin α1b

刊名：Neuroscience

出版年：2017

3.ERα inhibited myocardin-induced differentiation in uterine fibroids

作者：Xing-Hua Liao^a ; ^b ; ¹ ; ^{xinghualiao@hotmail.com} ; Jun-Yan Li^d ; ¹ ; Xiu-Mei Dong^a ; ^f ; Xiuhong Wang^e ; Yuan Xiang^a ; Hui Li^a ; Cheng-Xi Yu^a ; Jia-Peng Li^a ; Bai-Yin Yuan^a ; Jun Zhou^a ; ^c ; ^{zhoujun@wust.edu.cn} ; Tong-Cun Zhang^a ; ^b ; ^{zhangtongcun@wust.edu.cn}

关键词：USMC ; uterine smooth muscle cells ; α-SMA ; alpha smooth muscle actin ; PBS ; phosphate-buffered saline ; H&E ; hematoxylin and eosin ; DAPI ; 4&prime ; 6&prime ; -diamidino-2-phenylindole ; RT-PCR ; reverse-transcription polymerase chain reaction ; qRT-PCR ; quantitative real-time RT-PCR ; ChIP ; chromatin immunoprecipitation ; SERMs ; selective estrogen receptor modulators ; ERE ; estrogen receptor binding element ; ER ; estrogen receptors

刊名：Experimental Cell Research

出版年：2017

4.Identification and evaluation of reference genes for expression studies by RT-qPCR during embryonic development of the emerging model organism, Macrobrachium olfersii

作者：Michael L. Jaramillo^a ; ¹ ; Dib Ammar^a ; ^b ; ¹ ; Ruth L. Quispe^c ; Frank Guzman^d ; Rogerio Margis^e ; Evelise M. Nazari^a ; ² ; ^{evelise.nazari@ufsc.br} ; Yara M.R. Mü ; ller^a ; ²

关键词：RT-qPCR ; real-time reverse transcription quantitative polymerase chain reaction ; cDNA ; complementary DNA ; MIQE ; Minimum Information for Publication of Quantitative Real-Time PCR Experiments ; β-act ; β-actin ; GAPDH ; glyceraldehyde-3-phosphate dehydrogenase ; RpL8 ; ribosomal protein L8 ; RpS6 ; ribosomal protein S6 ; EF-1α ; elongation factor-1α ; AK ; arginine kinase ; M ; muscle ; HPT ; hepatopancreas ; CG ; cerebral ganglia ; Cq ; quantification cycle ; Ct ; threshold cycle ; E ; efficiency ; NFs ; normalization fac

刊名：Gene

出版年：2017

5.Transient receptor potential melastatin (TRPM) 8 is expressed in freshly isolated native human odontoblasts

作者：Kento Tazawa ; Hideharu Ikeda ; ^{hms-ikeda.endo@tmd.ac.jp} ; Nobuyuki Kawashima ; Takashi Okiji

关键词：DAPI ; 4&prime ; 6-diamidino-2-phenylindole ; DMP1 ; dentin matrix protein 1 ; DSPP ; dentin sialophosphoprotein ; GAPDH ; glyceraldehyde 3-phosphate dehydrogenase ; TRP ; transient receptor potential ; TRPA1 ; transient receptor potential ankyrin 1 ; TRPM8 ; transient receptor potential melastatin 8 ; PCR ; polymerase chain reaction ; qRT-PCR ; quantitative reverse transcription polymerase chain reaction ; RT-PCR ; reverse transcription polymerase chain reaction

刊名：Archives of Oral Biology

出版年：2017

6.Identification and functional analysis of pointed homologs in Bombyx mori

作者：Hiromitsu Tanaka ; ^{htanaka1@affrc.go.jp} ; Aki Sagisaka

关键词：B. mori ; Bombyx mori ; BmPNTs ; B. mori Pointeds ; AMP ; antimicrobial peptides ; Imd ; the immune deficiency ; shRNA ; short hairpin RNA ; PCR ; polymerase chain reaction ; RT-PCR ; reverse transcription-PCR ; qRT-PCR ; quantitative reverse transcriptase-PCR ; ORF ; open reading frame ; RACE ; rapid amplification of the cDNA ends ; RP ; ribosomal protein ; MAPK ; mitogen-activated protein kinase ; PGRP ; peptidoglycan recognition protein

刊名：Gene

出版年：2017

7.Geographic variation in RNAi sensitivity in the migratory locust

作者：Ryohei Sugahara ; ^{rsugahara@affrc.go.jp} ; ^{fancryo@yahoo.co.jp} ; Seiji Tanaka ; Akiya Jouraku ; Takahiro Shiotsuki

关键词：RNAi ; RNA interference ; Crz ; corazonin ; dsRNA ; double-stranded RNA ; MD ; Minami-Daito Island ; IB ; Ibaraki ; TS ; Tsushima Island ; HK ; Hokkaido Island ; EcR ; ecdysone receptor ; Ago ; Argonaute ; Dcr ; Dicer ; SID-1 ; systemic RNAi defective-1 ; BLAST ; basic local alignment search tool ; semi-qRT-PCR ; semi-quantitative reverse transcription-PCR

刊名：Gene

出版年：2017

8.Reference gene validation for relative quantification analysis of transcripts in urinary exfoliated cells among urothelial bladder carcinoma patients

作者：Mandana Afsharpad^a ; Mohammad Reza Nowroozi^b ; Mojtaba Safari^a ; Leila Nekoohesh^c ; Ahmad sohrabi^d ; Kazem Zendehdel^a ; ^e ; Mohammad Hossein Modarressi^a ; ^f ; ^{modaresi@tums.ac.ir} ; ^{fateme.karami@gmail.com}

关键词：UEC ; urinary exfoliated cells ; RT-qPCR ; Reverse Transcription-quantitative Polymerase Chain Reaction ; HKGs ; housekeeping genes ; ACTB ; Actin Beta ; GAPDH ; Glyceraldehyde-3-phosphate dehydrogenase ; SDHA ; subunit A of Succinate dehydrogenase complex ; TBP ; TATA box binding protein ; UBC ; Ubiquitin C ; HPRT1 ; Hypoxanthine phosphoribosyltransferase 1 ; RPS13 ; Ribosomal protein S13 ; HSP90AB1 ; Heat shock protein 90 ; kDa alpha ; PGM1 ; Phosphoglucomutase 1 ; BPH ; benign prostatic hyperplasia ; PBS ; Phosphate

刊名：Meta Gene

出版年：2017

9.Cigarette smoke extract induces epithelial-mesenchymal transition of human bladder cancer T24 cells through activation of ERK1/2 pathway

作者：Xin Sun^a ; ¹ ; Qifei Deng^b ; ¹ ; Zhaofeng Liang^c ; Zhiqi Liu^a ; Hao Geng^a ; Li Zhao^a ; Qirui Zhou^d ; Jie Liu^a ; Jiaxing Ma^a ; Daming Wang^a ; Dexin Yu^a ; ^{yudx_urology@126.com} ; Caiyun Zhong^e ; ^{cyzhong@njmu.edu.cn}

关键词：CSE ; Cigarette smoke extract ; CS ; Cigarette smoke ; EMT ; Epithelial&ndash ; mesenchymal transition ; MAPK ; Mitogen-activated protein kinase ; ERK1/2 ; extracellular regulated protein kinases 1 and 2 ; AP-1 ; activator protein 1 ; DMSO ; dimethyl sulfoxide ; MTT ; thiazolyl blue tetrazolium bromide ; qRT-PCR ; quantitative reverse transcription-polymerase chain reaction

刊名：Biomedicine & Pharmacotherapy

出版年：2017

10.Surfactant protein A deficiency exacerbates renal interstitial fibrosis following obstructive injury in mice

作者：Shaojiang Tian^a ; ^b ; ¹ ; ^{sc229@uga.edu} ; Chenxiao Li^a ; ¹ ; Ran Ran^a ; ^b ; Shi-You Chen^a ; ^b ;

关键词：SP-A ; surfactant protein A ; UUO ; unilateral ureter obstruction ; HMGB1 ; high mobility group box 1 ; TGF-β ; transforming growth factor β ; BMM ; bone marrow derived-macrophages ; HPTC ; human proximal tubular cell ; qRT-PCR ; Reverse transcription and quantitative RT-PCR ; Co-IP ; co-immunoprecipitation ; α-SMA ; smooth muscle α-actin ; FSP-1 ; fibroblast specific protein 1 ; IL-6 ; Interleukin 6

刊名：Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease

出版年：2017

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