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1.Deciphering, Communicating, and Engineering the CRISPR PAM

作者：Ryan T. Leenay ; Chase L. Beisel ; ^{cbeisel@ncsu.edu}

关键词：CRISPR ; clustered regularly interspaced short palindromic repeat ; Cas ; CRISPR-associated ; PAM ; protospacer-adjacent motif ; crRNAs ; CRISPR RNAs ; PFS ; protospacer-flanking sequence ; rPAM ; RNA PAM

刊名：Journal of Molecular Biology

出版年：2017

2.Characteristics of Genome Editing Mutations in Cereal Crops

作者：Changfu Zhu¹ ; ⁶ ; Luisa Bortesi² ; ⁶ ; Can Baysal¹ ; Richard M. Twyman³ ; Rainer Fischer² ; ⁴ ; Teresa Capell¹ ; Stefan Schillberg⁴ ; Paul Christou¹ ; ⁵ ; ^{christou@pvcf.udl.cat}

关键词：clustered regularly interspaced short palindromic repeats (CRISPRs) ; designer nuclease ; meganuclease ; plant genome editing ; transcription activator-like effector nucleases (TALENs) ; targeted mutation ; zinc-finger endonucleases (ZFNs)

刊名：Trends in Plant Science

出版年：2017

3.Genomic translational research: Paving the way to individualized cardiac functional analyses and personalized cardiology

作者：Ares Pasipoularides¹ ; ^{apasipou@duke.edu}

关键词：Personalized/precise medicine ; DNA microarrays ; Next-generation genome sequencing (NGS) ; Genome-wide association studies (GWAS) ; CRISPR-Cas9 genetic&ndash ; phenotypic screen ; Molecular genomic decoding of phenotypic diversity ; Omics-based tests ; Molecular genetic biomarkers ; Pharmacogenomics and pharmacogenetics

刊名：International Journal of Cardiology

出版年：2017

4.Zebrafish Genome Engineering Using the CRISPR-Cas9 System

作者：Mingyu Li¹ ; ⁵ ; Liyuan Zhao² ; ⁵ ; Patrick S. Page-McCaw³ ; ⁴ ; ^{patrick.s.page-mccaw@vanderbilt.edu" class="auth_mail" title="E-mail the corresponding author} ; Wenbiao Chen³ ; ^{wenbiao.chen@vanderbilt.edu" class="auth_mail" title="E-mail the corresponding author}

关键词：CRISPR&ndash ; Cas9 ; genome editing ; zebrafish ; knockout ; knock-in ; genetic screen

刊名：Trends in Genetics

出版年：2016

5.Controlling transcription in human pluripotent stem cells using CRISPR-effectors

作者：Ryan M. Genga ; Nicola A. Kearns ; René ; Maehr ; ^{rene.maehr@umassmed.edu" class="auth_mail" title="E-mail the corresponding author}

关键词：CAG ; hybrid of cytomegalovirus immediate early enhancer ; chicken β-actin promoter and intron ; rabbit β-globin splice acceptor ; Cas ; CRISPR-associated ; CRISPR ; clustered regularly interspaced short palindromic repeats ; crRNA ; CRISPR-derived RNA ; dCas9 ; nuclease-dead Cas9 ; hPSC ; human pluripotent stem cell ; KRAB ; Krueppel repressor associated box ; mESC ; mouse embryonic stem cell ; PAM ; protospacer adjacent motif ; sgRNA ; single guide RNA ; Sp ; Streptococcus pyogenes ; tracrRNA ; trans-activating CRISPR

刊名：Methods

出版年：2016

6.Characterization of CRISPR-Cas system in clinical Staphylococcus epidermidis strains revealed its potential association with bacterial infection sites

关键词：CRISPR-Cas ; Staphylococcus epidermidis ; Phage 6ec ; Spacer ; Evolution

刊名：Microbiological Research

出版年：2016

7.Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology

作者：Barbara Jusiak¹ ; ³ ; Sara Cleto¹ ; ³ ; Pablo Perez-Piñ ; era² ; Timothy K. Lu¹ ; ^{tim@lugroup.org" class="auth_mail" title="E-mail the corresponding author}

关键词：synthetic biology ; gene circuits ; CRISPR&ndash ; Cas9 ; synthetic transcription factors

刊名：Trends in Biotechnology

出版年：2016

8.Highly efficient CRISPR mutagenesis by microhomology-mediated end joining in Aspergillus fumigatus

作者：Chi Zhang ; Xiuhua Meng ; Xiaolei Wei ; Ling Lu ; ^{linglu@njnu.edu.cn" class="auth_mail" title="E-mail the corresponding author}

关键词：CRISPR mutagenesis ; Microhomology ; In situ tag-insertion ; Multi-locus genomic mutagenesis

刊名：Fungal Genetics and Biology

出版年：2016

9.Efficient genomic correction methods in human iPS cells using CRISPR-Cas9 system

作者：Hongmei Lisa Li^a ; ¹ ; Peter Gee^a ; Kentaro Ishida^a ; ^b ; Akitsu Hotta^a ; ^b ; ^{akitsu.hotta@cira.kyoto-u.ac.jp" class="auth_mail" title="E-mail the corresponding author}

关键词：iPS cells ; CRISPR ; Genome editing ; Knock-out ; Knock-in ; Gene correction

刊名：Methods

出版年：2016

10.Use of CRISPR-cas9 gene targeting for genome-scale CRISPR screening in a glioma stem-cell line

作者：Dr Imran Noorani ; BA MBBChir^a ; ^b ; ^{in1@sanger.ac.uk" class="auth_mail" title="E-mail the corresponding author} ; Prof Allan Bradley ; FRS^a

刊名：The Lancet

出版年：2016

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