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2.A bimolecular affinity purification method under denaturing conditions for rapid isolation of a ubiquitinated protein for mass spectrometry analysis
关键词：Nature Protocols ; protocols ; methods ; laboratory ; methodology ; techniques ; analytical chemistry ; antibodies ; biochemistry ; bioinformatics ; biological chemistry ; biotechnology ; biophysics ; cell biology ; chemistry ; computational biology ; computational chemistry ; conservation biology ; DNA sequencing ; developmental biology ; drug discovery ; ecology ; electrophysiology ; endocrinology ; evolutionary biology ; forensics ; genetics ; gene expression ; genomics ; glycomics ; histology ; immunology ; marine biology ; metabolomics/metabonomics ; microarrays ; microbiology ; microscopy ; molecular biology ; mutagenesis ; nanotechnology ; neuroscience ; parasitology ; pharmacology ; physiology ; plant science ; protein engineering ; protein synthesis ; proteomics ; purification ; RNA isolation ; recombinant DNA ; recombinant proteins ; sequencing ; spectroscopy ; structural biology ; systems biology ; toxicology ; transduction ; transfection ; zoology ; nature journals ; science research ; science and nature ; cell signalling ; science research papers ; rational drug design ; research papers ; nature ; animal cells ; plant biology ; proteins ; science experiments ; stem cell research ; biomedical ; life sciences ; biology articles ; science and technology ; nature magazines ; science ; biology projects.
刊名：Nature Protocols
出版年：2010
3.High-stringency tandem affinity purification of proteins conjugated to ubiquitin-like moieties
关键词：Nature Protocols ; protocols ; methods ; laboratory ; methodology ; techniques ; analytical chemistry ; antibodies ; biochemistry ; bioinformatics ; biological chemistry ; biotechnology ; biophysics ; cell biology ; chemistry ; computational biology ; computational chemistry ; conservation biology ; DNA sequencing ; developmental biology ; drug discovery ; ecology ; electrophysiology ; endocrinology ; evolutionary biology ; forensics ; genetics ; gene expression ; genomics ; glycomics ; histology ; immunology ; marine biology ; metabolomics/metabonomics ; microarrays ; microbiology ; microscopy ; molecular biology ; mutagenesis ; nanotechnology ; neuroscience ; parasitology ; pharmacology ; physiology ; plant science ; protein engineering ; protein synthesis ; proteomics ; purification ; RNA isolation ; recombinant DNA ; recombinant proteins ; sequencing ; spectroscopy ; structural biology ; systems biology ; toxicology ; transduction ; transfection ; zoology ; nature journals ; science research ; science and nature ; cell signalling ; science research papers ; rational drug design ; research papers ; nature ; animal cells ; plant biology ; proteins ; science experiments ; stem cell research ; biomedical ; life sciences ; biology articles ; science and technology ; nature magazines ; science ; biology projects.
刊名：Nature Protocols
出版年：2010
4.Affinity purification of plasmid DNA by temperature-triggered precipitation
关键词：Nature Protocols ; protocols ; methods ; laboratory ; methodology ; techniques ; analytical chemistry ; antibodies ; biochemistry ; bioinformatics ; biological chemistry ; biotechnology ; biophysics ; cell biology ; chemistry ; computational biology ; computational chemistry ; conservation biology ; DNA sequencing ; developmental biology ; drug discovery ; ecology ; electrophysiology ; endocrinology ; evolutionary biology ; forensics ; genetics ; gene expression ; genomics ; glycomics ; histology ; immunology ; marine biology ; metabolomics/metabonomics ; microarrays ; microbiology ; microscopy ; molecular biology ; mutagenesis ; nanotechnology ; neuroscience ; parasitology ; pharmacology ; physiology ; plant science ; protein engineering ; protein synthesis ; proteomics ; purification ; RNA isolation ; recombinant DNA ; recombinant proteins ; sequencing ; spectroscopy ; structural biology ; systems biology ; toxicology ; transduction ; transfection ; zoology ; nature journals ; science research ; science and nature ; cell signalling ; science research papers ; rational drug design ; research papers ; nature ; animal cells ; plant biology ; proteins ; science experiments ; stem cell research ; biomedical ; life sciences ; biology articles ; science and technology ; nature magazines ; science ; biology projects.
刊名：Nature Protocols
出版年：2007
5.High-throughput assay for determining specificity and affinity of protein-DNA binding interactions
关键词：Nature Protocols ; protocols ; methods ; laboratory ; methodology ; techniques ; analytical chemistry ; antibodies ; biochemistry ; bioinformatics ; biological chemistry ; biotechnology ; biophysics ; cell biology ; chemistry ; computational biology ; computational chemistry ; conservation biology ; DNA sequencing ; developmental biology ; drug discovery ; ecology ; electrophysiology ; endocrinology ; evolutionary biology ; forensics ; genetics ; gene expression ; genomics ; glycomics ; histology ; immunology ; marine biology ; metabolomics/metabonomics ; microarrays ; microbiology ; microscopy ; molecular biology ; mutagenesis ; nanotechnology ; neuroscience ; parasitology ; pharmacology ; physiology ; plant science ; protein engineering ; protein synthesis ; proteomics ; purification ; RNA isolation ; recombinant DNA ; recombinant proteins ; sequencing ; spectroscopy ; structural biology ; systems biology ; toxicology ; transduction ; transfection ; zoology ; nature journals ; science research ; science and nature ; cell signalling ; science research papers ; rational drug design ; research papers ; nature ; animal cells ; plant biology ; proteins ; science experiments ; stem cell research ; biomedical ; life sciences ; biology articles ; science and technology ; nature magazines ; science ; biology projects.
刊名：Nature Protocols
出版年：2006
6.Tandem affinity purification of functional TAP-tagged proteins from human cells
关键词：Nature Protocols ; protocols ; methods ; laboratory ; methodology ; techniques ; analytical chemistry ; antibodies ; biochemistry ; bioinformatics ; biological chemistry ; biotechnology ; biophysics ; cell biology ; chemistry ; computational biology ; computational chemistry ; conservation biology ; DNA sequencing ; developmental biology ; drug discovery ; ecology ; electrophysiology ; endocrinology ; evolutionary biology ; forensics ; genetics ; gene expression ; genomics ; glycomics ; histology ; immunology ; marine biology ; metabolomics/metabonomics ; microarrays ; microbiology ; microscopy ; molecular biology ; mutagenesis ; nanotechnology ; neuroscience ; parasitology ; pharmacology ; physiology ; plant science ; protein engineering ; protein synthesis ; proteomics ; purification ; RNA isolation ; recombinant DNA ; recombinant proteins ; sequencing ; spectroscopy ; structural biology ; systems biology ; toxicology ; transduction ; transfection ; zoology ; nature journals ; science research ; science and nature ; cell signalling ; science research papers ; rational drug design ; research papers ; nature ; animal cells ; plant biology ; proteins ; science experiments ; stem cell research ; biomedical ; life sciences ; biology articles ; science and technology ; nature magazines ; science ; biology projects.
刊名：Nature Protocols
出版年：2007
7.Purification of antibodies using the synthetic affinity ligand absorbent MAbsorbent A2P
关键词：Nature Protocols ; protocols ; methods ; laboratory ; methodology ; techniques ; analytical chemistry ; antibodies ; biochemistry ; bioinformatics ; biological chemistry ; biotechnology ; biophysics ; cell biology ; chemistry ; computational biology ; computational chemistry ; conservation biology ; DNA sequencing ; developmental biology ; drug discovery ; ecology ; electrophysiology ; endocrinology ; evolutionary biology ; forensics ; genetics ; gene expression ; genomics ; glycomics ; histology ; immunology ; marine biology ; metabolomics/metabonomics ; microarrays ; microbiology ; microscopy ; molecular biology ; mutagenesis ; nanotechnology ; neuroscience ; parasitology ; pharmacology ; physiology ; plant science ; protein engineering ; protein synthesis ; proteomics ; purification ; RNA isolation ; recombinant DNA ; recombinant proteins ; sequencing ; spectroscopy ; structural biology ; systems biology ; toxicology ; transduction ; transfection ; zoology ; nature journals ; science research ; science and nature ; cell signalling ; science research papers ; rational drug design ; research papers ; nature ; animal cells ; plant biology ; proteins ; science experiments ; stem cell research ; biomedical ; life sciences ; biology articles ; science and technology ; nature magazines ; science ; biology projects.
刊名：Nature Protocols
出版年：2007
8.Titanium dioxide as a chemo-affinity solid phase in offline phosphopeptide chromatography prior to HPLC-MS/MS analysis
关键词：Nature Protocols ; protocols ; methods ; laboratory ; methodology ; techniques ; analytical chemistry ; antibodies ; biochemistry ; bioinformatics ; biological chemistry ; biotechnology ; biophysics ; cell biology ; chemistry ; computational biology ; computational chemistry ; conservation biology ; DNA sequencing ; developmental biology ; drug discovery ; ecology ; electrophysiology ; endocrinology ; evolutionary biology ; forensics ; genetics ; gene expression ; genomics ; glycomics ; histology ; immunology ; marine biology ; metabolomics/metabonomics ; microarrays ; microbiology ; microscopy ; molecular biology ; mutagenesis ; nanotechnology ; neuroscience ; parasitology ; pharmacology ; physiology ; plant science ; protein engineering ; protein synthesis ; proteomics ; purification ; RNA isolation ; recombinant DNA ; recombinant proteins ; sequencing ; spectroscopy ; structural biology ; systems biology ; toxicology ; transduction ; transfection ; zoology ; nature journals ; science research ; science and nature ; cell signalling ; science research papers ; rational drug design ; research papers ; nature ; animal cells ; plant biology ; proteins ; science experiments ; stem cell research ; biomedical ; life sciences ; biology articles ; science and technology ; nature magazines ; science ; biology projects.
刊名：Nature Protocols
出版年：2007
9.Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site–specific stable isotope tagging
关键词：Nature Protocols ; protocols ; methods ; laboratory ; methodology ; techniques ; analytical chemistry ; antibodies ; biochemistry ; bioinformatics ; biological chemistry ; biotechnology ; biophysics ; cell biology ; chemistry ; computational biology ; computational chemistry ; conservation biology ; DNA sequencing ; developmental biology ; drug discovery ; ecology ; electrophysiology ; endocrinology ; evolutionary biology ; forensics ; genetics ; gene expression ; genomics ; glycomics ; histology ; immunology ; marine biology ; metabolomics/metabonomics ; microarrays ; microbiology ; microscopy ; molecular biology ; mutagenesis ; nanotechnology ; neuroscience ; parasitology ; pharmacology ; physiology ; plant science ; protein engineering ; protein synthesis ; proteomics ; purification ; RNA isolation ; recombinant DNA ; recombinant proteins ; sequencing ; spectroscopy ; structural biology ; systems biology ; toxicology ; transduction ; transfection ; zoology ; nature journals ; science research ; science and nature ; cell signalling ; science research papers ; rational drug design ; research papers ; nature ; animal cells ; plant biology ; proteins ; science experiments ; stem cell research ; biomedical ; life sciences ; biology articles ; science and technology ; nature magazines ; science ; biology projects.
刊名：Nature Protocols
出版年：2006
10.Isothermal titration calorimetry to determine association constants for high-affinity ligands
关键词：Nature Protocols ; protocols ; methods ; laboratory ; methodology ; techniques ; analytical chemistry ; antibodies ; biochemistry ; bioinformatics ; biological chemistry ; biotechnology ; biophysics ; cell biology ; chemistry ; computational biology ; computational chemistry ; conservation biology ; DNA sequencing ; developmental biology ; drug discovery ; ecology ; electrophysiology ; endocrinology ; evolutionary biology ; forensics ; genetics ; gene expression ; genomics ; glycomics ; histology ; immunology ; marine biology ; metabolomics/metabonomics ; microarrays ; microbiology ; microscopy ; molecular biology ; mutagenesis ; nanotechnology ; neuroscience ; parasitology ; pharmacology ; physiology ; plant science ; protein engineering ; protein synthesis ; proteomics ; purification ; RNA isolation ; recombinant DNA ; recombinant proteins ; sequencing ; spectroscopy ; structural biology ; systems biology ; toxicology ; transduction ; transfection ; zoology ; nature journals ; science research ; science and nature ; cell signalling ; science research papers ; rational drug design ; research papers ; nature ; animal cells ; plant biology ; proteins ; science experiments ; stem cell research ; biomedical ; life sciences ; biology articles ; science and technology ; nature magazines ; science ; biology projects.
刊名：Nature Protocols
出版年：2006
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