The Huangshandong mafic-ultramafic intrusion, is located at the centre segment of Tudun-Huangshan-Jing’erquan- Tulaergen mafic-ultramafic rock belt, the northern of the East Tianshan, controlled by the Kangurtag- Huangshan ductile sheer zone, and it is a multiple intrusion formed by at least three pulses of magmas with different compositions, and composes of lherzolite, olivine gabbro, gabbro norite, and gabbro diorite. Sulfide mineralization is associated with the second (gabbronorite) and third (plagioclase-bearing lherzolite-wehrlite) intrusive phases that are volumetrically minor in the intrusion. The Hf and Sr-Nd isotopic compositions of the Huangshandong intrusion are significantly different from that of the Tarim basalts and coeval mafic-ultramafic dykes in the Tarim Basin, similarly with the volcanic arc basalts and consistent with the mid-ocean basalts (MORB). The results of mixing calculations using Sr-Nd isotopes show that crustal contamination is <2% in the Huangshandong parental magmas.