The feature of major elements and REEs composition of lake sediments of Yaoshang Formation in Weining County, Guizhou Province, are analyzed. The provenance features, climatic evolution and control factors of sediment accumulation have been discussed. The results show that: The change of abundance of Al, Mg, Ca, K, Na and Ti is consistent in the section. Their correlations between 0.522 and 0.985 are very high. Fe and Mn form a positive correlation, while they negatively correlate with Al, Mg, Ca, K, Na and Ti. The abundance of Fe2O3 in the yellow clay of iron layer is very high. The chondrite-normalized REE distribution patterns of samples are similar with that of soil and red clay of karst areas in Guizhou Province, almost similar with that of weathering crust of carbonate rocks in Guizhou Province, and are inconsistent with Emeishan basalt.