According to forward and retrieval comprehensive analysis on the data of carbon isotope, pyrolysis of source rocks, reservoir analysis, pressure of gas reservoir, fluid inclusions of Yinan 2 gas reservoir in Kuqa depression, Tarim Basin, the genetic type of this gas reservoir was discriminated. The results show that the densification of sandstone reservoirs of Jurassic Ahe Formation in Yinan 2 gas reservoir took place about 11 Ma ago, while hydrocarbon expulsion peak of source rocks in this reservoir dated from 5 Ma ago. The authors point out that Yinan 2 gas reservoir is a large-scale deep-basin gas reservoir in the early stage, while adjusts and modifies later, which leads to the gas-bearing range controlled by south-north fault zones.