Rapid analysis and quantification of major neutral lipid species and free fatty acids by HPLC-ELSD from microalgae

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• 作者：Florentin Donot ; Caroline Strub ; Angé ; lique Fontana ; Nicolas Jouy ; Christelle Delbes ; Ziya Gunata and Sabine Schorr-Galindo
• 刊名：European Journal of Lipid Science and Technology
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：118
• 期：10
• 页码：1550-1556
• 全文大小：653K
• ISSN：1438-9312

文摘

A method based on normal-phase high performance liquid chromatography combined with evaporative light-scattering detector was developed to quantify major neutral lipids from microalgae. A binary gradient enabled a good resolution of hydrocarbons and major neutral lipids by classes in less than 13 min. A good linearity was observed from 0.9 to 30 μg. Limits of detection (LOD) and quantification (LOQ) was from 3 to 64 ng depending on the analyte. The intra- and inter-run precision (% RSD) of analysis was found less than 10%. The method was successfully applied to microalgae lipid extracts from Botryococcus braunii, Chlorella vulgaris, Spirulina platensis, and Porphyridium cruentum. B. braunii was distinguished by the highest levels of lipids, triacylglycerol (TAGs), and diacylglycerol (DAGs) being the major compounds.