Plant-specific DUF1110 protein from Oryza sativa: expression, purification and crystallization

详细信息    查看全文

• 作者：Kenichi Harada ; Eiki Yamashita ; Kento Inoue ; Koji Yamaguchi ; Toshimichi Fujiwara ; Atsushi Nakagawa ; Tsutomu Kawasaki and Chojiro Kojima
• 关键词：plant-specific protein ; PAMP-triggered immunity ; interactor for OsPUB44 ; Os01T0156300 ; Oryza sativa ; DUF1110
• 刊名：Acta Crystallographica Section F
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：72
• 期：6
• 页码：480-484
• 全文大小：745K
• ISSN：1744-3091

文摘

The Os01T0156300 protein from Oryza sativa has been classified into the domain of unknown function (DUF) family DUF1110. DUF1110 family members exist in monocotyledons but not in dicotyledons, and share no sequence identity with proteins for which structures have been reported. In this study, the Os01T0156300 protein was crystallized using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 1.84 Å resolution. The crystal belonged to space group P2₁, with unit-cell parameters a = 89.9, b = 89.8, c = 107.1 Å, β = 106.6°. The asymmetric unit was estimated to contain 6–11 molecules.