文摘
This study was designed to investigate the potential reproductive health benefits of Raphanus sativus L. (radish) sprout ethanolic extract (RSE) in a bisphenol A (BPA)-exposed rat model of testicular disorders and to identify the bioactive components of RSE. RSE and its butanolic layer (RSE-BuOH) exhibited the strongest total antioxidant capacity, as determined using the oxygen radical absorbance capacity method. The RSE-BuOH was fractionated using chromatography with active guided fractionation, and 10 major compounds were identified in gas chromatography-mass spectrometry analysis. Among them, 3-hexyl-1-cyclopentene and 2-cyclopentene-1-tridecanoic acid were confirmed to be the major components. In addition, administration of RSE significantly restored body weight, testes weight and sperm cell rate in BPA-exposed rats. In particular, administration of RSE-BuOH to BPA-exposed rats significantly increased sperm cell rate and serum testosterone level. These results suggest that RSE may attenuate reproductive dysfunction caused by BPA-induced oxidative stress in male rats.
