Lentiviral expression of GAD67 and CCK promoter-driven opsins to target interneurons in vitro and in vivo

详细信息    查看全文

• 作者：Laura Mantoan Ritter ; Douglas C. Macdonald ; Georg Ritter ; David Escors ; Francesca Chiara ; Anna Cariboni ; Stephanie Schorge ; Dimitri M. Kullmann and Mary Collins
• 关键词：adenoassocated virus (AAV) ; gene - delivery ; microscopy ; neuroscience ; retroviruses ; virology - lentiviruses
• 刊名：The Journal of Gene Medicine
• 出版年：2016
• 出版时间：January-March 2016
• 年：2016
• 卷：18
• 期：1-3
• 页码：27-37
• 全文大小：2078K
• ISSN：1521-2254

文摘

The ability to manipulate the activity of interneurons with optogenetic tools offers the possibility of interfering with diseases caused by altered neuronal inhibition and synchrony, including epilepsy and schizophrenia. To develop vectors for therapeutic approaches, targeting optogenetic constructs to interneurons is therefore a key requirement. We investigated whether the interneuron-specific promoters glutamic acid decarboxylase (GAD)67 and cholecystokinin (CCK) allowed targeted lentiviral delivery of opsins to interneurons as a whole, or specifically CCK+ interneurons.