Targeting expression to megakaryocytes and platelets by lineage-specific lentiviral vectors

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• 作者：L. J. Latorre-Rey ; S. Wintterle ; S. Dü ; tting ; S. Kohlscheen ; T. Abel ; F. Schenk ; S. Wingert ; M. A. Rieger ; B. Nieswandt ; N. Heinz and U. Modlich
• 刊名：Journal of Thrombosis and Haemostasis
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：15
• 期：2
• 页码：341-355
• 全文大小：1654K
• ISSN：1538-7836

文摘

Lentiviral transduction and transplantation of hematopoietic stem cells (HSCs) can be utilized to modify the phenotype of megakaryocytes and platelets. As the genetic modification in HSCs is transmitted onto all hematopoietic progenies, transgene expression from the vector should be restricted to megakaryocytes to avoid un-physiologic effects by ectopic transgene expression. This can be achieved by lentiviral vectors that control expression by lineage-specific promoters.