Ultrahigh-resolution Fourier transform ion cyclotron resonance mass spectrometry and tandem mass spectrometry for peptide de novo amino acid sequencing for a seven-protein mixture by paired single-residue transposed Lys-N and Lys-C digestion

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• 作者：Xiaoyan Guan ; Naomi C. Brownstein ; Nicolas L. Young and Alan G. Marshall
• 刊名：Rapid Communications in Mass Spectrometry
• 出版年：2017
• 出版时间：30 January 2017
• 年：2017
• 卷：31
• 期：2
• 页码：207-217
• 全文大小：1971K
• ISSN：1097-0231

文摘

Bottom-up tandem mass spectrometry (MS/MS) is regularly used in proteomics to identify proteins from a sequence database. De novo sequencing is also available for sequencing peptides with relatively short sequence lengths. We recently showed that paired Lys-C and Lys-N proteases produce peptides of identical mass and similar retention time, but different tandem mass spectra. Such parallel experiments provide complementary information, and allow for up to 100% MS/MS sequence coverage.