Directional Regulation of Enzyme Pathways through the Control of Substrate Channeling on a DNA Origami Scaffold

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• 作者：Guoliang Ke ; Dr. Minghui Liu ; Shuoxing Jiang ; Dr. Xiaodong Qi ; Yuhe Renee Yang ; Shaun Wootten ; Dr. Fei Zhang ; Prof. Zhi Zhu ; Prof. Yan Liu ; Prof. Chaoyong James Yang and Prof. Hao Yan
• 刊名：Angewandte Chemie
• 出版年：2016
• 出版时间：June 20, 2016
• 年：2016
• 卷：128
• 期：26
• 页码：7609-7612
• 全文大小：2456K
• ISSN：1521-3757

文摘

Artificial multi-enzyme systems with precise and dynamic control over the enzyme pathway activity are of great significance in bionanotechnology and synthetic biology. Herein, we exploit a spatially addressable DNA nanoplatform for the directional regulation of two enzyme pathways (G6pDH–MDH and G6pDH–LDH) through the control of NAD⁺ substrate channeling by specifically shifting NAD⁺ between the two enzyme pairs. We believe that this concept will be useful for the design of regulatory biological circuits for synthetic biology and biomedicine.