Over-expression of (1,3;1,4)-β-D-glucanase isoenzyme EII gene results in decreased (1,3;1,4)-β-D-glucan content and increased starch level in barley grains

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• 作者：Ning Han ; Chenglong Na ; Yuqiong Chai ; Jianshu Chen ; Zhongbo Zhang ; Bin Bai ; Hongwu Bian ; Yuhong Zhang and Muyuan Zhu
• 刊名：Journal of the Science of Food and Agriculture
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：97
• 期：1
• 页码：122-127
• 全文大小：514K
• ISSN：1097-0010

文摘

High content of (1,3;1,4)-β-d-glucan in barley grains is regarded as an undesirable factor affecting malting potential, brewing yield and feed utilization. Production of thermostable bacterial (1,3;1,4)-β-glucanase in transgenic barley grain or supplementation of exogenous bacterial (1,3;1,4)-β-glucanase has been used to improve malt and feed quality. The aim of the present study was to investigate the effect of over-expression of an endogenous (1,3;1,4)-β-glucanase on β-glucan content and grain composition in barley.