刊名:Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
出版年:2017
出版时间:February 2017
年:2017
卷:1862
期:2
页码:167-175
全文大小:1619 K
卷排序:1862
文摘
The coding sequence of E. siliculosus CYP5164B1 enzyme has been expressed in E. coli. The recombinant 477 AA protein preferentially utilized linoleate 9-hydroperoxide. Enzyme produced the epoxyalcohols like 9,10-epoxy-11-hydroxy-12-octadecenoic acid. Both 18O atoms from [18O2]9-hydroperoxide largely incorporated into epoxyalcohol. CYP5164B1 is a first CYP74-related enzyme of oxylipin biosynthesis in brown algae.
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