Parameter’s optimization and kinetics study of α-amylase enzyme of Bacillus sp. MB6 isolated from vegetable waste

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• 作者：Jai Shankar Paul^a ; B.M. Lall^b ; S.K. Jadhav^a ; ^{jadhav9862@gmail.com} ; K.L. Tiwari^a
• 关键词：3 ; 5-dinitrosalicylic acid (PubChem CID ; 11873) ; Acrylamide (PubChem CID ; 6579) ; Magnesium sulphate (PubChem CID ; 24083) ; Starch (PubChem CID ; 439341) ; Calcium chloride (PubChem CID ; 5284359) ; Magnesium sulphate (PubChem CID ; 24083) ; Dipotassium hydrogen phosphate (PubChem CID ; 24450) ; Sodium dodecyl sulphate (PubChem CID ; 3423265) ; Coomassie brilliant blue (PubChem CID ; 6333920)
• 刊名：Process Biochemistry
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：52
• 期：Complete
• 页码：123-129
• 全文大小：814 K
• 卷排序：52

文摘

Isolation of bacteria from vegetable waste for amylase enzyme production. Optimization of enzyme production and activity condition. Immobilization and reuse of immobilized enzyme can reduce cost of the product. Purified enzyme showed very high specific activity against substrate. Enzyme kinetics was studied to check rate of reaction against substrate.