Functional expression, production, and biochemical characterization of a laccase using yeast surface display technology

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• 作者：Brandt Bertrand ; Marí ; a R. Trejo-Hern&aacute ; ndez ; Daniel Morales-Guzm&aacute ; n ; Luis Caspeta ; Ramó ; n Su&aacute ; rez Rodrí ; guez ; Fernando Martí ; nez-Morales ; ^{fernandomm@uaem.mx}
• 关键词：Laccase immobilization ; Saccharomyces cerevisiae EBY100 ; Medium optimization ; Whole-cell biocatalyst
• 刊名：Fungal Biology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：120
• 期：12
• 页码：1609-1622
• 全文大小：2606 K
• 卷排序：120

文摘

Functional expression of laccase in yeast surface display system. Laccase production was increased 6.5-fold by medium optimization. Laccase heterologous expression surpassed production by native strain. High thermal stability of biologically immobilized laccase. Biologically immobilized laccase presented high reuse capacity.