Label-free detection of DNA molecules moving in micro-fluidic channels by infrared absorption spectroscopy

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• 作者：Tomoyuki Miyoshi^a ; ¹Author Vitae ; Ken-ichi Ishibashi^a ; ²Author Vitae ; Koichiro Miyamoto^bAuthor Vitae ; Ayumi Hirano-Iwata^c ; ^dAuthor Vitae ; Yasuo Kimura^d ; ^eAuthor Vitae ; Michio Niwano^a ; ^{niwano@riec.tohoku.ac.jp" class="auth_mail" title="E-mail the corresponding author}Author Vitae
• 关键词：Micro-fluidic channel ; DNA ; Infrared spectroscopy
• 刊名：Sensors & Actuators: B. Chemical
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：238
• 期：Complete
• 页码：917-922
• 全文大小：1772 K

文摘

Infrared absorption spectroscopy (IRAS) in a multiple internal reflection (MIR) geometry is integrated with silicon-based micro-fluidic channels to allow detection and separation of DNA molecules in the micro-channels. The micro-channels were fabricated on a Si MIR-prism using the conventional silicon lithographic techniques. In the present method we can probe solution-phase biomolecular species through IR spectral profiles of those biomolecules, allowing us to detect and separate biomolecules without fluorescence labeling. The applicability of this approach is demonstrated with a representative test case: transport of single-stranded DNA molecules (oligonucleotides) in the micro-fluidic channels.