Identification and characterization of a novel amidase signature family amidase from Parvibaculum lavamentivorans ZJB14001

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Identification and characterization of a novel amidase signature family amidase from Parvibaculum lavamentivorans ZJB14001

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作者：Zhe-Ming Wu^a ; ^b ; Ren-Chao Zheng^a ; ^b ; Yu-Guo Zheng^a ; ^b ; ^{zhengyg@zjut.edu.cn" class="auth_mail" title="E-mail the corresponding author}
关键词：Amidase signature family ; Cloning ; Substrate specificity ; Parvibaculum lavamentivorans
刊名：Protein Expression and Purification
出版年：2017
出版时间：January 2017
年：2017
卷：129
期：Complete
页码：60-68
全文大小：2020 K

文摘

•: A novel amidase signature (AS) amidase (Pl-Ami) was identified by genome mining.
•: The recombinant Pl-Ami were purified to homogeneity and characterized in detail.
•: The narrow substrate spectrum of Pl-Ami distinguish it from other AS amidases.
•: Pl-Ami preferred to hydrolyze long chain aliphatic amides.

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