Controllable surface groups’ density of MNCs was tuned by adjusting the ratio of APTS-to-MNCs.
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Excess APTS input exhibited negative influence on antibody conjugation and MICTS detection.
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Steric hindrance of highly branch conformation between surface groups from excess APTS input was found.
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The controlled surface functionalization of MNCs is the key for highly efficient and sensitive ICTS.
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