Glycosylation and antiproliferative activity of hyperglycosylated IFN-α2 potentiate HEK293 cells as biofactories

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• 作者：Agustina Gugliotta^a ; Natalia Ceaglio^a ; Brenda Raud^b ; Guillermina Forno^b ; ^c ; Laura Mauro^c ; Ricardo Kratje^a ; Marcos Oggero^a ; ^{moggero@fbcb.unl.edu.ar}
• 关键词：Hyperglycosylated IFN-α2b ; N-glycosylation ; Glycoproteins ; Antitumoral activity ; Pharmacokinetics ; HEK293 cells ; CHO cells
• 刊名：European Journal of Pharmaceutics and Biopharmaceutics
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：112
• 期：Complete
• 页码：119-131
• 全文大小：1144 K
• 卷排序：112

文摘

Hyperglycosylated human IFN-α2 was produced in CHO and HEK cells. CHO-derived IFN showed highly sialylated and branched glycans. HEK-derived IFN displayed smaller and truncated glycans compared to CHO-derived one. Differences in the glycosylation pattern had not a considerable impact on the elimination half-life. Hyperglycosylated IFN produced in HEK was more efficient as an antiproliferative agent in vitro. IFN4NHEK showed a higher in vivo antitumor activity in animal models.