Human herpesvirus 6 (HHV-6) U94/REP protein inhibits betaherpesvirus replication

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• 作者：Elisabetta Caselli ; Arianna Bracci ; Monica Galvan ; Michela Boni ; Antonella Rotola ; Carlo Bergamini ; Claudio Cermelli ; Paola Dal Monte ; Ursula A. Gompels ; Enzo Cassai and Dario Di Luca
• 关键词：HHV-6 ; U94/rep gene ; Betaherpesvirus inhibition
• 刊名：Virology
• 出版年：2006
• 出版时间：15 March 2006
• 年：2006
• 卷：346
• 期：2
• 页码：402-414
• 全文大小：1078 K

文摘

Human herpesvirus 6 (HHV-6) is the only human herpesvirus encoding U94/rep, homologue to the parvovirus non-structural gene rep68/78. Results to date suggest that HHV-6 U94/rep might regulate viral gene expression and have a role in viral latency. To determine the effect of U94/REP upon viral replication, the protein was produced. The purified U94/REP retained the characteristic immunological features. It was internalized and localized in the nucleus of human cells, showing marked inhibitory activity on the replication of HHV-6 (both variants A and B). The effect of U94/REP was dose-dependent and sensitive to treatment with single-stranded but not double-stranded DNA. U94/REP inhibited the replication of other betaherpesviruses, HHV-7 and human cytomegalovirus, but had no effect on herpes simplex virus. These results confirm the action of U94/rep latency gene in the regulation of HHV-6 replication with implications for co-reactivations and latency of human betaherpesviruses.