文摘
To elucidate the mechanism of mature embryo dedifferentiation in wheat (Triticum aestivum L.), the activity of genes in the dedifferentiating embryos of wheat cultivar Yumai 18 was determine using Affymetrix microarray technique. The wheat embryos were cultured on MS medium supplemented with 2 mg L−1 of 2,4-dichlorophenolxyacetic acid (2,4-D) and sampled at 2, 6, 12, 24, and 72 h after inoculation. With the help of online tools at NCBI, DATF, and DRTF websites, 80 auxin-related genes were found to be differentially expressed during embryo dedifferentiation, including 41 up-regulated, 29 down-regulated, and 10 both up- and down-regulated genes. The up-regulated genes were more than the down-regulated ones, especially at 24 h and 72 h after inoculation. These genes were supposed to be involved in several biological processes, such as the transportation, response, induction, synthesis, and degeneration of auxin. The expressions of 7 genes (BG906698, BQ281752, CD454626, CD864552, CD938626, BJ233383, and AY543630) were examined and analyzed using semiquantitative technique. The expression patterns of these genes were consistent with the gene expression profiling obtained by Affymetrix microarray technique. Gene of plasma membrane (PM) H+-ATPase (GenBank accession number: AY543630) was expressed in a high level at 2 h after inoculation, and then decreased to the minimum level at 24 h after inoculation. This result implied that PM H+-ATPase gene might be a trigger for dedifferentiation of mature embryos in wheat.
