Expression and characterization of immunodominant region of fusion protein of peste des petits ruminants virus in E. coli

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• 作者：R. Apsana<sup>asup><sup> ; sup><sup>bsup> ; V. Balamurugan<sup>bsup><sup> ; sup><sup>b.vinayagamurthy@icar.gov.insup><sup> ; sup><sup>balavirol@gmail.comsup> ; B.M. Veeregowda<sup>asup> ; S. Abraham<sup>bsup> ; D.S.N. Raju<sup>bsup> ; D. Rathnamma<sup>asup> ; S.M. Byregowda<sup>csup> ; H. Rahman<sup>bsup><sup> ; sup><sup>dsup> ; M.S. Shaila<sup>esup>
• 关键词：PPRV ; Immunodominant ectodomain ; F protein ; E. coli ; Expression ; Purification ; Immunoreactivity
• 刊名：Small Ruminant Research
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：144
• 期：Complete
• 页码：75-82
• 全文大小：1167 K
• 卷排序：144

文摘

High level expression of immunodominant ectodomain of PPRVF protein in E. coli in native form. Its characterization and immunoreactivity as antigen or immunogen by ELISA and immunoblotting. Immune response of the expressed recombinant protein in Rabbit was studied. Expressed recombinant PPRV F protein can be used for detection of PPRV antibodies in infected and vaccinated sheep and goats.