Improving the activity of surface displayed cytochrome P450 enzymes by optimizing the outer membrane linker

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• 作者：Paul Quehl^a ; ^{paul.quehl@uni-muenster.de} ; Jan Schü ; ü ; rmann^a ; ^{jan.schueuermann@uni-muenster.de} ; Joel Hollender^a ; ^b ; ^{mail.hollender@gmail.com} ; Joachim Jose^a ; ^{joachim.jose@uni-muenster.de}
• 关键词：BAM ; β-barrel assembly machinery ; CPR ; Cytochrome P450 reductase ; CtxB ; Cholera toxine subunit B ; CYP ; Cytochrome P450 monooxygenase ; NADP+ ;   ; Nicotinamide adenine dinucleotide phosphate ; FAD ; flavin adenine dinucleotide ; FMN ; flavin mononucleotide ; ORF ; open reading frame ; OM ; Outer membrane ; OMPI ; Outer membrane protein isolation ; LB ; lysogeny broth
• 刊名：Biochimica et Biophysica Acta (BBA) - Biomembranes
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：1859
• 期：1
• 页码：104-116
• 全文大小：2143 K
• 卷排序：1859

文摘

Surface display of human cytochrome P450 reductase and cytochrome P450 1A2 Comprehensive study about the effects of engineering the linker of the outer membrane anchored autotransporter EhaA and AIDA-I Whole cell activity can be substantially enhanced by engineering the linker between the passenger and the β-barrel of the autotransporter