A p53-type response element in the GDF15 promoter confers high specificity for p53 activation

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• 作者：Motonobu Osada ; Hannah Lui Park ; Min Joo Park ; Jun-Wei Liu ; Guojun Wu ; Barry Trink ; David Sidransky
• 关键词：p53 response element ; GDF15 ; Promoter ; p53 transactivation ; Gene activation
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2007
• 出版时间：23 March 2007
• 年：2007
• 卷：354
• 期：4
• 页码：913-918
• 全文大小：662 K

文摘

GDF15 is a transcriptional target gene for p53 and its family members, p63 and p73. Its promoter region contains two p53-type response elements, RE1 and RE2, and RE2 confers p53-specific transactivation. RE2 contains several mismatches from the canonical p53 response element (RRRCWWGYYY). Two mismatches in the RRR span and T base of the RE2 core sequence in the most 3′ quarter-site are critical for inhibiting the binding affinity to p63 and p73 and corresponding promoter activity. Our results strongly suggest that differential DNA-binding affinities between p53 family member proteins act, at least in part, to confer specific target gene activation.