1D NMR methods for determination of degree of cross-linking and BDDE substitution positions in HA hydrogels

详细信息    查看全文

• 作者：Frida J. Wende<sup>asup><sup> ; sup><sup>Frida.Wende@slu.sesup> ; Suresh Gohil<sup>asup><sup> ; sup><sup>Suresh.Gohil@slu.sesup> ; Lars I. Nord<sup>bsup><sup> ; sup><sup>Lars.Nord@galderma.comsup> ; Anne Helander Kenne<sup>bsup><sup> ; sup><sup>Anne.HelanderKenne@galderma.comsup> ; Corine Sandströ ; m<sup>asup><sup> ; sup><sup>Corine.sandstrom@slu.sesup>
• 关键词：HA ; hyaluronic acid/hyaluronan ; MoD ; degree of modification ; CrR ; effective crosslinker ratio ; BDDE ; 1 ; 4-butanediol diglycidyl ether ; BDPE ; 1 ; 4-butanediol di-(propan-2 ; 3-diolyl)etherch ; ABC ; chondroitinase ABC ; GlcNAc ; N-acetyl-d-glucoseamine ; ΔGlcA ; d-glucuronic acid with C4-C5 unsaturation ; NOE ; nuclear overhauser effect ; SEC ; size exclusion chromatography ; TDA ; triple detector array
• 刊名：Carbohydrate Polymers
• 出版年：2017
• 出版时间：10 February 2017
• 年：2017
• 卷：157
• 期：Complete
• 页码：1525-1530
• 全文大小：1306 K
• 卷排序：157

文摘

1D carbon NMR method to determine the degree of cross-linking in HA hydrogels was developed. 1D proton NMR method to determine the cross-linker position in HA hydrogels was developed. No chromatographic separation step is required prior to NMR analysis.