RHA-P: Isolation, expression and characterization of a bacterial α-l-rhamnosidase from Novosphingobium sp. PP1Y

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• 作者：Federica De Lise^a ; ¹ ; Francesca Mensitieri^a ; ¹ ; Vincenzo Tarallo^b ; Nicola Ventimiglia^a ; Roberto Vinciguerra^b ; Annabella Tramice^c ; Roberta Marchetti^b ; Elio Pizzo^a ; Eugenio Notomista^a ; Valeria Cafaro^a ; Antonio Molinaro^b ; Leila Birolo^b ; Alberto Di Donato^a ; Viviana Izzo^d ; ^{vizzo@unisa.it}
• 关键词：α-RHAs ; α-l-rhamnosidases ; GHs ; glycosyl hydrolases ; GTs ; glycosyl transferases ; PPMM ; potassium phosphate minimal medium ; pNPR ; p-nitrophenyl-α-l-rhamnopyranoside ; MOPS ; 3-(N-morpholino) propanesulfonic acid ; pNP ; p-nitrophenolate ; BSA ; bovine serum albumin ; LB ; Luria Bertani medium ; LB-N ; Luria Bertani medium containing a final concentration of 0.5&thinsp ; M NaCl ; LB-BS ; Luria Bertani medium supplemented with 1&thinsp ; mM of both betaine and sorbitol ; LB-NBS ; Luria Bertani medium containing a fi
• 刊名：Journal of Molecular Catalysis B: Enzymatic
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：134
• 期：part_PA
• 页码：136-147
• 全文大小：2004 K
• 卷排序：134

文摘

Isolation and recombinant expression of a bacterial α-RHA from Novosphingobium sp. PP1Y was performed. Mechanism of action, kinetic parameters, pH and temperature optimum, tolerance to organic solvents of RHA-P were evaluated. The enzyme showed a promising biotechnological potential and was able to hydrolyze natural flavonoids.