Reference gene validation for relative quantification analysis of transcripts in urinary exfoliated cells among urothelial bladder carcinoma patients

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• 作者：Mandana Afsharpad^a ; Mohammad Reza Nowroozi^b ; Mojtaba Safari^a ; Leila Nekoohesh^c ; Ahmad sohrabi^d ; Kazem Zendehdel^a ; ^e ; Mohammad Hossein Modarressi^a ; ^f ; ^{modaresi@tums.ac.ir} ; ^{fateme.karami@gmail.com}
• 关键词：UEC ; urinary exfoliated cells ; RT-qPCR ; Reverse Transcription-quantitative Polymerase Chain Reaction ; HKGs ; housekeeping genes ; ACTB ; Actin Beta ; GAPDH ; Glyceraldehyde-3-phosphate dehydrogenase ; SDHA ; subunit A of Succinate dehydrogenase complex ; TBP ; TATA box binding protein ; UBC ; Ubiquitin C ; HPRT1 ; Hypoxanthine phosphoribosyltransferase 1 ; RPS13 ; Ribosomal protein S13 ; HSP90AB1 ; Heat shock protein 90  ; kDa alpha ; PGM1 ; Phosphoglucomutase 1 ; BPH ; benign prostatic hyperplasia ; PBS ; Phosphate
• 刊名：Meta Gene
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：11
• 期：Complete
• 页码：36-42
• 全文大小：515 K
• 卷排序：11

文摘

Systematic validation of reference genes has been performed for RT-qPCR analysis of urinary exfoliated cells in this study. BestKeeper, geNorm, and NormFinder indicated similar results for the two most stable HKGs in favor of GAPDH and HSP90AB1. ACTB found to be the least stable one by all three algorithms. Among all nine studied HKGs only GAPDH, HSPCB, and UBC delivered appropriate stability, according to M values of geNorm algorithm (< 1.5). Using two of these HKGs would normalize target gene's expression levels more effectively based on BestKeeper algorithm.