Enhancing cognate target elution efficiency in gel-free chemical proteomics

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Enhancing cognate target elution efficiency in gel-free chemical proteomics

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作者：Branka Radic-Sarikas^a ; ³ ; Uwe Rix^a ; ^b ; ³ ; Alexey Stukalov^a ; ¹ ; Manuela Gridling^a ; André ; C. Mü ; ller^a ; Jacques Colinge^a ; ² ; Giulio Superti-Furga^a ; ^c ; Keiryn L. Bennett^a ; ^{kbennett@cemm.oeaw.ac.at" class="auth_mail" title="E-mail the corresponding author}
关键词：MS ; mass spectrometry ; LC ; liquid chromatography ; LCMS ; liquid chromatography mass spectrometry ; iTRAQ ; isobaric tags for relative and absolute quantitation ; SDS ; sodium dodecyl sulphate ; CML ; chronic myeloid leukemia ; TEA ; triethylamine ; HPLC-MS ; high-performance liquid chromatography mass spectrometry ; TEAB ; triethylammonium bicarbonate ; FA ; formic acid ; ACN ; acetonitrile ; XX-NHS ; biotin-biotinamidohexanoyl-6-aminohexanoic acid N-hydroxysuccinimide ester ; DTT ; dithiothreitol ; TLCK ; N-alpha-tos
刊名：EuPA Open Proteomics
出版年：2015
出版时间：December 2015
年：2015
卷：9
期：Complete
页码：43-53
全文大小：3005 K

文摘

•: 5">A new elution strategy for LCMS coupled to chemical proteomics is shown.
•: Method is beneficial for both drug immobilization protocols and biotinylated drugs.
•: 5">We observed enhanced target recovery without enrichment of non-specific proteins.
•: This was not constrained neither to particular targets, nor to a single drug.

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