Usefulness of PKHs for studying cell proliferation

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• 作者：Boutonnat ; Jean ; Barbier ; Magali ; Rousselle ; Cé ; cile ; Muirhead ; Katharine A. ; Mousseau ; Mireille ; et. al.
• 关键词：PKH26 ; dye dilution ; proliferation ; hematological malignancies ; flow cytometry ; PKH26 ; intercalant membranaire ; prolifé ; ration cellulaire ; pathologie hé ; matologique
• 刊名：Comptes Rendus de l'Academie des Sciences Series III Sciences de la Vie
• 出版年：1998
• 出版时间：November, 1998
• 年：1998
• 卷：321
• 期：11
• 页码：901-907
• 全文大小：536 K

文摘

Classical methods for proliferative assessment (such as tritiated thymidine or bromodeoxyuridine (BrdUrd) incorporation) need sample fixation. As an alternative, we have evaluated the use of a dye dilution method using PKH26 to determine the rate and extent of proliferation in cell lines. Flow cytometric analysis associated with modelling software makes it possible to estimate the number of cells having undergone different numbers of cell divisions by sampling the cell population at varying times post-labelling. Two major questions were addressed in these studies. (i) Does PKH26 give a stable and reproducible labelling? (ii) Does labelling with PKH26 alter cellular proliferation characteristics? We conclude that the methods developed here provide a simpler, more complete means for assessment of cell proliferation in patients with hematological malignancies.