Kinesin-Dependent Axonal Transport Is Mediated by the Sunday Driver (SYD) Protein

详细信息    查看全文

• 作者：Bowman ; Aaron B. ; Kamal ; Adeela ; Ritchings ; Bruce W. ; Philp ; Alastair Valentine ; McGrail ; Maura ; et. al.
• 刊名：Cell
• 出版年：2000
• 出版时间：October 10, 2000
• 年：2000
• 卷：103
• 期：4
• 页码：583-594
• 全文大小：1.21 M

文摘

A broadly conserved membrane-associated protein required for the functional interaction of kinesin-I with axonal cargo was identified. Mutations in sunday driver (syd) and the axonal transport motor kinesin-I cause similar phenotypes in Drosophila, including aberrant accumulations of axonal cargoes. GFP-tagged mammalian SYD localizes to tubulovesicular structures that costain for kinesin-I and a marker of the secretory pathway. Coimmunoprecipitation analysis indicates that mouse SYD forms a complex with kinesin-I in vivo. Yeast two-hybrid analysis and in vitro interaction studies reveal that SYD directly binds kinesin-I via the tetratricopeptide repeat (TPR) domain of kinesin light chain (KLC) with K_d 200 nM. We propose that SYD mediates the axonal transport of at least one class of vesicles by interacting directly with KLC.