The effect of the surface charge of hydrogel supports on thermophilic biohydrogen production

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• 作者：Maria Assunta Basile ; Laura Dipasquale ; Agata Gambacorta ; Monica Filomena Vella ; Anna Calarco ; Pierfrancesco Cerruti ; Mario Malinconico ; Giovanna Gomez d’ ; Ayala
• 关键词：Microbiological hydrogen production ; Thermophiles ; Immobilization ; Polymer hydrogels ; Surface charge
• 刊名：Bioresource Technology
• 出版年：2010
• 出版时间：June 2010
• 年：2010
• 卷：101
• 期：12
• 页码：4386-4394
• 全文大小：446 K

文摘

The effect of the surface charge of different immobilizing hydrogels on biohydrogen production in batch cultures was investigated using a novel isolate associated to the genus Thermoanaerobacterium. Two crosslinked polysaccharide-based hydrogels and two acrylic hydrogels were tested as polymeric carriers for cell adsorption. Immobilization improved both substrate conversion and hydrogen cumulative production compared to the suspended culture, and a yield of 1.9 mol H₂/mol glucose was observed after 24 h for alginate-supported cultures. Cationic carriers dramatically increased cell immobilization, leading to markedly faster kinetics of substrate degradation and hydrogen production in batch operation, with a peak of 3.6 mol H₂/mol glucose for the acrylic hydrogel HM92. Accumulation of gaseous and acidic metabolites inhibited further H₂ production, shifting the carbon flow to reduced end-products and biomass synthesis. Preliminary tests showed that all the tested hydrogels had good durability and allowed hydrogen production on repeated batch runs.