Production of yellow fever virus in microcarrier-based Vero cell cultures

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• 作者：Marta Cristina O. Souza ; Marcos S. Freire ; Erica A. Schulze ; Luciane P. Gaspar ; Leda R. Castilho
• 关键词：Yellow fever virus ; Microcarrier-based culture ; Vero cells ; Serum-free medium
• 刊名：Vaccine
• 出版年：2009
• 出版时间：30 October 2009
• 年：2009
• 卷：27
• 期：46
• 页码：6420-6423
• 全文大小：380 K

文摘

In this work, the propagation of the 17DD yellow fever virus in Vero cells grown on Cytodex-1 microcarriers was evaluated. After verifying that upon infection the virus adsorption step could be performed under continuous agitation, experiments were carried out in spinners and sparged lab-scale stirred-tank bioreactor to evaluate the use of a commercial serum-free medium (VP-SFM) and to investigate the effects of multiplicity of infection (MOI) and time of infection (TOI) on virus production. Virus titers as high as 8.4 × 10⁸ pfu/mL were obtained upon infection with MOI of 0.02 and TOI of 3 days, using the serum-free medium in the sparged bioreactor.