Carbamazepine treatment recovered low N-acetylaspartate+N-acetylaspartylglutamate (tNAA) levels in the megencephaly mouse BALB/cByJ-Kv1.1^mceph/mceph

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• 作者：Eric Westman ; Christian Spenger ; Lars-Olof Wahlund ; Catharina Lavebratt
• 关键词：MRS ; LCModel ; PLS-DA ; Epilepsy ; mceph/mceph ; Carbamazepine
• 刊名：Neurobiology of Disease
• 出版年：2007
• 出版时间：April 2007
• 年：2007
• 卷：26
• 期：1
• 页码：221-228
• 全文大小：569 K

文摘

Megencephaly mice (BALB/cByJ-Kv1.1^mceph/mceph) display excessive brain growth and seizures related to a mutation within the potassium channel gene Kv1.1 producing a malfunctioning protein. ¹H Magnetic resonance spectroscopy (MRS) provides means to study brain transmitters and metabolites in vivo. We applied MRS to pinpoint differences in hippocampus between mceph/mceph and wild type (wt) mice. Carbamazepine (CBZ) protects against brain overgrowth in mceph/mceph. Therefore, the effects of durable oral CBZ treatment on the MR spectra were investigated. LCModel was used for spectra quantification and multivariate data analysis applied to detect group differences. mceph/mceph mice had lower levels of N-acetylaspartate+N-acetylaspartylglutamate (tNAA) and choline-containing (tCho) compounds compared to wt mice. Glutamate, glutamine, taurine and myo-inositol levels were similar in wt and mceph/mceph. Furthermore, CBZ treatment recovered tCho and tNAA levels in mceph/mceph. Thus, distinct differences in MRS spectra between mceph/mceph and wt mice were depicted and treatment effects of CBZ were monitored using MRS.