Coated protein nanoclusters from influenza H7N9 HA are highly immunogenic and induce robust protective immunity

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• 作者：Li Wang ; PhD^a ; ^{lwang40@emory.edu} ; Timothy Z. Chang ; BS^b ; ^{tzchang@gatech.edu} ; Yuan He ; PhD^a ; ^{yuan.he3@emory.edu} ; Jong R. Kim ; PhD^a ; ^{jongrok.kim@emory.edu} ; Shelly Wang ; BS^a ; ^{swang59@emory.edu} ; Teena Mohan ; PhD^a ; ^c ; ^{mohan.teena@emory.edu} ; Zachary Berman ; BS^a ; ^c ; ^{zack.berman@emory.edu} ; S. Mark Tompkins ; PhD^d ; ^smt@uga.edu ; Ralph A. Tripp ; PhD^d ; ^{ratripp@uga.edu} ; Richard W. Compans ; PhD^a ; ^{rcompan@emory.edu} ; Julie A. Champion ; PhD^b ; ^{julie.champion@chbe.gatech.edu} ; Bao-Zhong Wang ; PhD^a ; ^c ; ^{bwang23@gsu.edu}
• 关键词：Influenza vaccine ; Pandemic influenza ; Protein nanoclusters
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：13
• 期：1
• 页码：253-262
• 全文大小：1170 K
• 卷排序：13

文摘

Recurring influenza viruses pose an annual threat to public health. A time-saving, cost-effective and egg-independent influenza vaccine approach is important particularly when responding to an emerging pandemic. We fabricated coated, two-layer protein nanoclusters from recombinant trimeric hemagglutinin from an avian-origin H7N9 influenza A virus as an approach for vaccine development in response to an emerging pandemic. Assessment of the virus-specific immune responses and protective efficacy in mice immunized with the nanoclusters demonstrated that the vaccine candidates were highly immunogenic, able to induce protective immunity and long-lasting humoral antibody responses to this virus without the use of adjuvants. Because the advantages of the highly immunogenic coated nanoclusters also include rapid productions in an egg-independent system, this approach has great potential for influenza vaccine production not only in response to an emerging pandemic, but also as a replacement for conventional seasonal influenza vaccines.