Purification and characterization of chondroitinase ABC from Acinetobacter sp. C26

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• 作者：Changliang Zhu^b ; ¹ ; ^{chlzhu@163.com} ; Jingliang Zhang^b ; ^c ; ¹ ; ^{jlzhang1985@163.com} ; Jing Zhang^a ; ^{zjys1991@163.com} ; Yanhui Jiang^a ; ^{shannongjyh@163.com} ; Zhaopeng Shen^b ; ^c ; ^{shenzhaopeng1987@163.com} ; Huashi Guan^b ; ^{sunyang@ouc.edu.cn} ; Xiaolu Jiang^a ; ^{jiangxl@ouc.edu.cn}
• 关键词：Chondroitinase ABC ; Acinetobacter ; Purification ; Characterization
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：95
• 期：Complete
• 页码：80-86
• 全文大小：1088 K
• 卷排序：95

文摘

Purification and chondroitinase ABC from Acinetobacter sp. C26 was carried out. Identification of purified chondroitinase ABC was determined. Characterisation of the enzyme was carried out with respect to pH, temperature and presence of metal ions and chemicals. The kinetic parameters of the enzyme were studied.