High glucose induces N-linked glycosylation-mediated functional upregulation and overexpression of Ca_v3.2 T-type calcium channels in neuroendocrine-like differentiated human prostate cancer cells

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• 作者：Kazuki Fukami^a ; Erina Asano^a ; Mai Ueda^a ; Fumiko Sekiguchi^a ; Shigeru Yoshida^b ; Atsufumi Kawabata^a ; ^{kawabata@phar.kindai.ac.jp}
• 关键词：Cav3.2 T-type calcium channel ; Prostate cancer ; Asparagine-linked glycosylation
• 刊名：Journal of Pharmacological Sciences
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：133
• 期：1
• 页码：57-60
• 全文大小：1023 K
• 卷排序：133

文摘

Given that Cav3.2 T-type Ca2+ channels were functionally regulated by asparagine (N)-linked glycosylation, we examined effects of high glucose on the function of Cav3.2, known to regulate secretory function, in neuroendocrine-like differentiated prostate cancer LNCaP cells. High glucose accelerated the increased channel function and overexpression of Cav3.2 during neuroendocrine differentiation, the former prevented by enzymatic inhibition of N-glycosylation and cleavage of N-glycans. Hyperglycemia thus appears to induce N-linked glycosylation-mediated functional upregulation and overexpression of Cav3.2 in neuroendocrine-like differentiated prostate cancer cells.